Impact of artificial root exudates on the bacterial community structure in bulk soil and maize rhizosphere
Impact of artificial root exudates on the bacterial community structure in bulk soil and maize rhizosphere
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DOI：
10.1016/s0038-0717(03)00179-2
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发表时间：
2003-09-01
影响因子：
9.7
通讯作者：
Guckert, A
中科院分区：
文献类型：
--
作者：
Baudoin, E;Benizri, E;Guckert, A
细菌密度，代谢特征和遗传结构进行了评估，以衡量土壤富集可溶性有机碳的细菌群落结构的影响。在天然玉米分泌物（葡萄糖、果糖、蔗糖、柠檬酸、乳酸、琥珀酸、丙氨酸、丝氨酸和谷氨酸）中检测所选分泌物，并以100 μ g/g/天的速率使用14天。此外，两个不同的碳/氮比（20.5和40.1），通过不同的羧酸和氨基酸的浓度，合成的解决方案进行了比较，以评估潜在的作用，有机氮的可用性。体外实验包括将渗出液溶液施用于散装土壤。在对照的情况下，仅加入蒸馏水。两种溶液均显著增加了细菌密度，并改变了Biolog（R）GN 2平板的氧化模式，而C/N比对这两个参数没有影响。通过核糖体基因间间隔区分析（RISA）测量的遗传结构也被有机改良剂一致地修改。氮素有效性水平导致不同的遗传结构。在第二个实验中，以前的渗出液解决方案（C/N 20.5）之一，适用于15天的老玉米植株，以确定根际微生物群落（原位实验）的渗出物的结构影响。细菌密度显著增加，但程度低于体外实验中发现的。代谢潜力和RISA配置文件也显着修改的有机富集。(C)2003 Elsevier Ltd.保留所有权利。
Bacterial densities, metabolic signatures and genetic structures were evaluated to measure the impact of soil enrichment of soluble organic carbon on the bacterial community structures. The exudates chosen were detected in natural maize exudates (glucose, fructose, saccharose, citric acid, lactic acid, succinic acid, alanine, serine and glutamic acid) and were used at a rate of 100 mug C g(-1) day(-1) for 14 days. Moreover two synthetic solutions with distinct carbon/nitrogen ratios (20.5 and 40.1), obtained by varying carboxylic and amino acids concentrations, were compared in order to evaluate the potential role of organic N availability. The in vitro experiment consisted of applying exudate solutions to bulk soil. In the case of the control, only distilled water was added. Both solutions significantly increased bacterial densities and modified the oxidation pattern of Biolog(R) GN2 plates with no effect of the C/N ratio on these two parameters. Genetic structure, measured by means of ribosomal intergenic spacer analysis (RISA), was also consistently modified by the organic amendments. N availability levels led to distinct genetic structures. In a second experiment, one of the previous exudate solutions (C/N 20.5) was applied to 15-day-old maize plants to determine the structural influence of exudates on the rhizosphere microbial community (in situ experiment). Bacterial densities were significantly increased, but to a lesser extent than had been found in the in vitro experiment. Metabolic potentials and RISA profiles were also significantly modified by the organic enrichment. (C) 2003 Elsevier Ltd. All rights reserved.