Blueberry stem extract and stem active components prevent blue light-emitting diode light-induced retinal photoreceptor cell damage in vitro
Blueberry stem extract and stem active components prevent blue light-emitting diode light-induced retinal photoreceptor cell damage in vitro
复制标题
蓝莓茎提取物及茎活性成分体外预防蓝光发光二极管光诱导的视网膜感光细胞损伤
DOI：
10.1093/bbb/zbad001
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发表时间：
2023
影响因子：
1.6
通讯作者：
Yamasaki M.
中科院分区：
文献类型：
--
作者：
Ogawa K.;Urata K.;Suzuki Y.;Sugamoto K.;Goto Y.;Nakayama T.;Nishiyama K.;Kunitake H.;Yamasaki M.
蓝光导致视网膜损伤，可能导致眼部疾病，如年龄相关性黄斑变性。本研究旨在探讨蓝莓茎提取物（BStEx）及其活性成分对蓝光致视网膜光感受器细胞损伤的保护作用。将在BStEx或组分存在下培养的光感受器细胞暴露于蓝光以诱导细胞损伤。BStEx、含有原花青素、绿原酸、儿茶素和表儿茶素的BStEx级分防止细胞损伤和/或抑制活性氧（ROS）的产生。此外，BStEx减少细胞凋亡和细胞死亡，并抑制p38丝裂原活化蛋白激酶和c-Jun N-末端激酶的磷酸化导致细胞凋亡的蓝光暴露。这些发现表明，BStEx和组分通过抑制MAPK磷酸化和ROS产生对蓝光诱导的感光细胞损伤发挥保护作用。
Blue light causes retinal damage that can lead to ocular diseases such as age-related macular degeneration. In this study, we determined the protective effect of blueberry stem extract (BStEx) and active components on blue light-emitting diode (LED) light-induced retinal photoreceptor cell damagein vitro. Photoreceptor cells cultured in the presence of BStEx or components were exposed to blue light to induce cell damage. BStEx, fractions of BStEx containing proanthocyanidins, chlorogenic acid, catechin, and epicatechin prevented the cell damage and/or inhibited the generation of reactive oxygen species (ROS). Furthermore, BStEx reduced apoptosis and cell death, and inhibited the phosphorylation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase leading to cellular apoptosis induced by blue light exposure. These findings suggest that BStEx and components exert a protective effect against blue light-induced photoreceptor cell damage through the inhibition of MAPK phosphorylation and ROS production.