The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses.
The effect of dam strain on the craniofacial morphogenesis of CL/Fr mouse fetuses.
复制标题
母鼠应变对 CL/Fr 小鼠胎儿颅面形态发生的影响。
DOI：
10.11501/3108116
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Nakata
中科院分区：
文献类型：
--
作者：
D. Martin;K. Nonaka;K. Yanagita;M. Nakata
应用胚胎移植技术和头影测量方法，研究了Dam应变对宫内颅面发育的影响以及对唇腭裂(CLP)敏感的CL/Fr系胚胎的严重程度。将早期囊胚的CL/Fr品系转移到相同的DAM品系和抗CLP的C57BLDAM品系。妊娠第18天，剖腹取出胎儿。检查胎儿CLP的自发发生率，并在剖腹手术后立即对每个胎儿的头面部复合体进行头影测量观察。比较两种DAM品系未患胎儿的背腹颅面大小和患CLP I的严重程度。结果表明：(1)CL/Fr丹株正常胎儿的颅面总体尺寸明显小于C57/BL丹株。在CL/Fr丹株中观察到的受感染胎儿的胎龄小于在C57BL丹株中观察到的胎儿，但株间差异不显着。20母猪的菌株对未受影响的胎儿的颅面大小有非常显著的影响。3)CL/Fr坝株CLP频率显著高于C57BL坝株。4)CL/Fr株感染胎儿的CLP严重程度明显高于C57BL株。这些结果表明，对CLP敏感的CL/Fr DAM株对胎儿颅面发育有抑制作用，且CL/Fr DAM株对胎儿唇裂的影响比耐CLP的C57BL DAM株更严重。因此，从胚胎移植到CL/Fr和C57BLdam品系的小鼠胚胎的CL/Fr品系的颅面形态发生与胚胎的基因型别一起，可能与dam品系的作用有关。
The embryo transfer technique and cephalometry were used to investigate the effect of dam strain in intrauterine craniofacial growth and the severity of cleft lip and palate (CLP) in a CLP-susceptible CL/Fr strain of embryos. The CL/Fr strain of embryos at early blastocyst stage was transferred to the same dam strain and to the CLP-resistant C57BL dam strain. On the 18th gestational day, each dam was laparotomized to take out the fetuses. The spontaneous incidence of CLP in the fetuses was checked and a cephalometric observation of the craniofacial complex of each fetus was done just after laparotomy. The dorsoventral craniofacial size of the unaffected fetuses and the severity of CLP i the affected ones were compared between both dam strains. The following results were obtained: 1) The overall craniofacial sizes of the unaffected fetuses observed in the CL/Fr dam strain were significantly smaller than those seen in the C57/BL dam strain. Those of the affected fetuses observed in the CL/Fr dam strain were smaller than those seen in the C57BL dam strain although the interstrain difference was not significant. 20 The dam strain had a highly significant effect on the craniofacial size of the unaffected fetuses. 3) The CLP frequency in the CL/Fr dam strain was significantly higher than that in the C57BL dam strain. 4) The severity of CLP in the affected fetuses observed in the CL/Fr dam strain was significantly more serious than that seen in the C57BL dam strain. These results indicated that the CLP-susceptible CL/Fr dam strain retarded the intrauterine craniofacial growth of the fetuses and that the cleft condition in the affected fetuses observed in the CL/Fr dam strain was more seriously affected than that seen in the CLP-resistant C57BL dam strain. Thus, it can be concluded that the effect of the dam strain played an important role in the craniofacial morphogenesis of the CL/Fr strain of mouse fetuses that developed from the embryo transferred to the CL/Fr and C57BL dam strains along with the genotype of embryos.