Chitosan and trimethyl chitosan chloride (TMC) as adjuvants for inducing immune responses to ovalbumin in mice following nasal administration
Chitosan and trimethyl chitosan chloride (TMC) as adjuvants for inducing immune responses to ovalbumin in mice following nasal administration
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DOI：
10.1016/j.jconrel.2007.05.025
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发表时间：
2007-08-28
影响因子：
10.8
通讯作者：
Pitaksuteepong, Tasana
中科院分区：
文献类型：
--
作者：
Boonyo, Worawan;Junginger, Hans E.;Pitaksuteepong, Tasana
评价了不同分子量的壳聚糖（Chi-P，MW= 2.7 × 10（5）g/mol和Chi-A，MW= 5.0 × 10（5）g/mol）和不同季铵化程度（DQ）的氯化三甲基壳聚糖（TMC）（包括TMC-20、TMC-40和TMC-60）作为佐剂诱导对卵清蛋白（OVA）的免疫应答。将溶液和明矾中的OVA用作对照。在第0、7和14天免疫BALB/c小鼠组。在第0、13和21天检查IgG和伊加滴度。结果发现，在第13天和第21天，Chi-A都能诱导比Chi-P更高的对OVA的IgG应答。在第13天，在TMC-40中的OVA诱导的IgG应答显著高于在溶液、Chi-P和TMC-60中。此外，TMC-40中的OVA比明矾中的OVA更能诱导IgG应答。尽管在第21天未观察到显著差异，但显示TMC-40中的OVA比TMC-20、TMC-60和溶液中诱导更高的IgG应答。伊加反应在第21天最明显。同样，Chi-A可以比Chi-P和TMC-40诱导最高的IgA应答引起更高的IgA应答。总之，这些发现表明壳聚糖的MW和TMC的DQ都影响免疫诱导水平。TMC-40显示为用于鼻内施用的最有效佐剂。(C)2007 Elsevier B.V保留所有权利。
Chitosan of different molecular weights (Chi-P, MW=2.7x 10(5) g/mol and Chi-A, MW=5.0x 10(5) g/mol) and trimethyl chitosan chloride (TMC) of various degree of quaternization (DQ) including TMC-20, TMC-40 and TMC-60 were evaluated as adjuvants for inducing of immune responses to ovalbumin (OVA). OVA in solution and in alum were used as controls. Groups of BALB/c mice were immunized on days 0, 7 and 14. The IgG and IgA titers were examined on days 0, 13 and 2 1. It was found that for both days 13 and 2 1, Chi-A could elicit higher IgG responses to OVA than Chi-P. On day 13, OVA in TMC-40 induced lgG responses significantly higher than that in solution, Chi-P and TMC-60. Moreover, OVA in TMC-40 could induce IgG responses higher than OVA in alum. Although a significant difference was not observed at day 21, OVA in TMC-40 was shown to induce higher IgG responses than that in TMC-20, TMC-60 and solution. The IgA responses were the most pronounced on day 2 1. Again, Chi-A could elicite higher IgA responses than Chi-P and TMC-40 induced the highest IgA responses. In conclusion, these findings demonstrate that both MW of chitosan and DQ of TMC influence the level of immune induction. TMC-40 shows to be the most potent adjuvant for intranasal administration. (C) 2007 Elsevier B.V All rights reserved.