Immunoglobulin secretion by mouse X human hybridomas: an approach for the production of anti-idiotype reagents useful in monitoring patients with B cell lymphoma.
Immunoglobulin secretion by mouse X human hybridomas: an approach for the production of anti-idiotype reagents useful in monitoring patients with B cell lymphoma.
复制标题
小鼠 X 人杂交瘤分泌免疫球蛋白：一种生产抗独特型试剂的方法，可用于监测 B 细胞淋巴瘤患者。
作者：
S. Brown;J. Dilley;R. Levy
肿瘤细胞取自结节性淋巴瘤（一种单克隆 B 细胞恶性肿瘤）患者。这些非 Ig 分泌淋巴瘤细胞与小鼠骨髓瘤细胞杂交。分泌人Ig的杂交瘤以高频率获得。选择一个克隆进行研究，它是人类重链和轻链的稳定生产者，但失去了小鼠轻链的生产。它分泌人类 IgM 五聚体。该克隆产生的 Ig 被纯化并用作生产抗独特型抗体的免疫原。这些抗体可识别淋巴瘤细胞的特定细胞表面 Ig，并已用于检测原始患者血液中携带独特型的细胞，甚至在疾病处于临床缓解期时也是如此。此外，可以在患者的血清中检测到游离的独特型。 Id+细胞和游离独特型的水平都与疾病活动相关，在复发时上升，在缓解时下降。
Tumor cells were obtained from a patient with nodular lymphoma, a monoclonal B cell malignancy. These non-Ig-secreting lymphoma cells were hybridized to mouse myeloma cells. Hybridomas secreting human Ig were obtained in high frequency. One clone, which was a stable producer of human heavy and light chains, but which had lost production of mouse light chains, was chosen for study. It secreted a human IgM pentamer. The Ig produced by this clone was purified and used as an immunogen for the production of anti-idiotype antibodies. These antibodies recognized the specific cell surface Ig of the lymphoma cells and have been used to detect idiotype-bearing cells in the blood of the original patient, even at times when the disease was in clinical remission. Moreover, free idiotype could be detected in the patient's serum. Both the levels of Id+ cells and free idiotype correlated with disease activity, rising in relapse and falling in remission.