A simple and sensitive impedimetric aptasensor for the detection of tumor markers based on gold nanoparticles signal amplification
A simple and sensitive impedimetric aptasensor for the detection of tumor markers based on gold nanoparticles signal amplification
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一种简单灵敏的基于金纳米粒子信号放大的肿瘤标志物检测阻抗适体传感器
DOI：
10.1016/j.talanta.2014.08.072
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发表时间：
2015-01-15
期刊：
影响因子：
6.1
通讯作者：
Li, Yuanjian
中科院分区：
文献类型：
--
作者：
Liu, Xi;Qin, Yun;Li, Yuanjian
建立了一种基于金纳米颗粒（AuNPs）信号放大的电化学阻抗感应传感器，用于肿瘤标志物（mucin 1蛋白，MUC1为模型）的超灵敏检测。设计的与MUC1适配体部分互补的cDNA被固定在金电极上。MUC1的检测可以通过将核酸适配体的结构从DNA/DNA双工切换到DNA/靶复合体来实现。用氧化还原探针[Fe(CN)(6)]（3-/4-）电化学阻抗分析（EIS）表征了电极界面特征的变化。MUC1蛋白的定量检测是通过电子传递阻力（Delta R-et）的变化来实现的。此外，通过cDNA与适体杂交，将修饰的AuNPs （Apt@AuNPs）偶联物引入电极，作为信号增强子。正如预期的那样，MUC1的检测灵敏度大大提高，这可能是由于MUC1在Apt@AuNPs修饰电极表面的特异性结合。该传感器的检测限低至0.1 nM，同时具有灵敏度高、选择性好等优点。本研究可为基于阻抗适体传感器的肿瘤标志物检测提供一个通用模型。(C) 2014 Elsevier B.V.版权所有
A simple and sensitive electrochemical impedimetric aptasensor based on gold nanoparticles (AuNPs) signal amplification was developed for the ultrasensitive detection of tumor markers (mucin 1 protein, MUC1 as a model). The designed cDNA, which is partly complementary with the aptamer of MUC1 was immobilized on the gold electrode. The detection of MUC1 could be carried out by virtue of switching structures of aptamers from DNA/DNA duplex to DNA/target complex. The change of the interfacial feature of the electrode was characterized by electrochemical impedance analysis (EIS) with the redox probe [Fe(CN)(6)](3-/4-). The quantitative detection of MUC1 protein was obtained from the changes of electron-transfer resistance (Delta R-et). Moreover, as the signal enhancer, the aptamer-modified AuNPs (Apt@AuNPs) conjugates was introduced on the electrode by the hybridization of cDNA with aptamer. As expected, the detection sensitivity for MUC1 was greatly improved, which may be due to the specific binding of MUC1 onto the surface of the Apt@AuNPs modified electrode. This proposed simple aptasensor has a low detection limit of 0.1 nM, and also exhibits several advantages of high sensitivity and good selectivity. This present work may provide a general model for the detection of tumor marker based on impedimetric aptasensor. (C) 2014 Elsevier B.V. All rights reserved.