A CORRELATED LIGHT AND ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMICAL STUDY OF CHOLINERGIC TERMINALS AND NEURONS IN THE RAT AMYGDALOID BODY WITH SPECIAL EMPHASIS ON THE BASOLATERAL AMYGDALOID NUCLEUS
A CORRELATED LIGHT AND ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMICAL STUDY OF CHOLINERGIC TERMINALS AND NEURONS IN THE RAT AMYGDALOID BODY WITH SPECIAL EMPHASIS ON THE BASOLATERAL AMYGDALOID NUCLEUS
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DOI：
10.1002/cne.902440110
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发表时间：
1986-02-01
影响因子：
2.5
通讯作者：
HEIMER, L
中科院分区：
文献类型：
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作者：
CARLSEN, J;HEIMER, L
用乙酰胆碱生物合成酶胆碱乙酰转移酶（ChAT）的免疫细胞化学定位方法，研究了大鼠杏仁基底外侧核（BL）的胆碱能神经支配。ChAT免疫反应（ChAT-IR）元素观察整个BL的形式细点和静脉曲张的纤维。电子显微镜显示免疫反应性斑点代表小的终末（0.3-1.2 μ m），其中大部分与未标记的树突轴或刺形成突触接触。不太频繁，ChAT-IR终端建立突触接触与大的神经元细胞体，其所有的特性的投射神经元定义的基础上的轴突投射到腹侧纹状体。ChAT-IR终末有时与小的神经元细胞体（包括ChAT-IR神经元）形成突触接触。ChAT-IR终扣含有不同大小的多形性透明小泡，并且绝大多数突触是对称型的。在BL的所有部分都观察到小的ChAT-IR神经元。虽然ChAT-IR细胞体的形状从典型的纺锤形到圆形变化很大，但大多数具有或多或少的椭圆形，其大直径为10-14 μ m。大多数ChAT阳性神经元呈放射状双极树突状，但也有多极细胞。ChAT-IR神经元包含一个锯齿状的核，这是经常偏心定位和周围的薄或中等薄的细胞质边缘。所获得的结果进行了讨论有关的准皮质组织的BL。
The cholinergic innervation of the rat basolateral amygdaloid nucleus (BL) was determined by the immunocytochemical localization of the acetylcholine biosynthetic enzyme, choline acetyltransferase (ChAT). ChAT-immunoreactive (ChAT-IR) elements were observed throughout the BL in the form of fine puncta and varicose fibers. Electron microscopy revealed that the immunoreactive puncta represented small terminals (0.3-1.2 .mu.m), most of which formed synaptic contacts with unlabeled dendritic shafts or spines. Less frequently, ChAT-IR terminals established synaptic contacts with large neuronal cell bodies, which had all the characteristics of projection neurons as defined on the basis of axonal projections to the ventral striatum. ChAT-IR terminals were sometimes seen to form synaptic contacts with small neuronal cell bodies, including those of ChAT-IR neurons. The ChAT-IR boutons contained pleomorphic clear vesicles of varying size, and the large majority of the synapses were of the symmetric type. Small ChAT-IR neurons were observed in all parts of the BL. Although the ChAT-IR cell bodies varied widely in shape from typical fusiform to round, most had a more or less oval shape with a major diameter of 10-14 .mu.m. Most of the ChAT-IR neurons seemed to display a radial bipolar dendritic pattern, but multipolar cells were also observed. The ChAT-IR neurons contained an indented nucleus, which was often eccentrically located and surrounded by a thin or moderately thin rim of cytoplasm. The results obtained are discussed in relation to a quasi-cortical organization of the BL.