Progesterone-mediated efflux of cytosolic chloride during the human sperm acrosome reaction.
Progesterone-mediated efflux of cytosolic chloride during the human sperm acrosome reaction.
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人精子顶体反应过程中黄体酮介导的胞质氯化物流出。
DOI：
10.1006/bbrc.1995.2197
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Meizel,S
中科院分区：
文献类型：
--
作者：
Turner,KO;Meizel,S
孕酮能够在体外启动哺乳动物精子顶体反应，并且被认为是体内这一重要受精事件的启动器。我们之前的研究表明，孕酮通过激活一个独特的类固醇受体/Cl -通道（类似于γ-氨基丁酸受体/Cl -通道），至少在一定程度上启动了人类精子顶体反应。本研究使用细胞内Cl -荧光探针6-甲氧基- n -乙基喹啉检测精子胞浆中Cl -的质变。我们证明孕酮可以介导γ-氨基丁酸受体/Cl -通道拮抗剂picrotoxin和(+)-bicuculline（也抑制顶体反应）抑制的人精子胞浆Cl -的快速瞬时降低。这些结果支持γ-氨基丁酸样受体/Cl -通道参与p介导的人顶体反应，并首次证明快速Cl -外排在该事件中起作用。
Progesterone is capable of initiating the mammalian sperm acrosome reaction in vitro and is a putative initiator of this essential fertilization event in vivo. Our previous work has suggested that progesterone initiates the human sperm acrosome reaction, at least in part, by activating a unique steroid receptor/Cl−channel resembling a γ-aminobutyric acidAreceptor/Cl−channel (γ-aminobutyric acidA-like receptor/Cl−channel). Here, the fluorescent intracellular Cl−probe, 6-methoxy-N-ethylquinolinium, was used to detect qualitative changes in sperm cytosolic Cl−. We demonstrate that progesterone can mediate a rapid transient decrease of human sperm cytosolic Cl−inhibitable by the γ-aminobutyric acidAreceptor/Cl−channel antagonists picrotoxin and (+)-bicuculline (which also inhibit the acrosome reaction). These results support the involvement of a γ-aminobutyric acidA-like receptor/Cl−channel in the P-mediated human acrosome reaction and are the first to demonstrate that a rapid Cl−efflux plays a role in that event.