Bosellia serrata-induced apoptosis is related with ER stress and calcium release
Bosellia serrata-induced apoptosis is related with ER stress and calcium release
复制标题
DOI：
10.1007/s12263-007-0072-z
复制
发表时间：
2008-02-01
影响因子：
3.5
通讯作者：
Chae, Han-Jung
中科院分区：
文献类型：
--
作者：
Kim, Hyung-Ryong;Kim, Myung-Sunny;Chae, Han-Jung
据报道，乳香树胶树脂（BS）已被证明具有抗肿瘤特性，也可能具有抗癌作用。本研究探讨了BS作为抗癌剂的潜力。BS提取物诱导HeLa人宫颈癌细胞凋亡，如通过两种凋亡分析，Hoechst染色和Annexin V/PI测定所证实的。在细胞凋亡途径中，研究了ER应激相关机制，以确定其在BS诱导的细胞凋亡中的作用。测定内质网应激蛋白的代表GRP 78和CHOP以及钙结合蛋白-钙蛋白酶的表达。结果显示，BS处理的细胞中GRP 78和CHOP的水平显著高于对照细胞，并且钙蛋白酶活性更强。这表明ER应激信号传导和细胞凋亡之间存在相关性，这表明BS-ER应激引发剂作为人宫颈癌的抗癌治疗剂的可能性。
It has been reported that the gum resin of Boswellia serrata (BS), which has been shown to have antiinflammatory properties, might also have anticancer effects. This study examined the potential of BS as an anticancer agent. The BS extract induces apoptosis in HeLa human cervical carcinoma cells, as confirmed by two apoptosis analyses, Hoechst staining and Annexin V/PI assay. Among the apoptosis pathways, the ER stress-associated mechanism was examined to determine its role in BS-induced apoptosis. The expression of GRP78 and CHOP, which are representatives of the ER stress proteins, and the calcium-binding protein-calpain were determined. The results showed significantly higher levels of both GRP78 and CHOP, and stronger calpain activity in the BS-treated cells than in the control cells. This shows that there is a correlation between ER stress signaling and apoptosis, which suggests the possibility of the BS-ER stress initiator as an anticancer therapeutic agent in human cervical carcinoma.