Identification of the Outer-Inner Dynein Linker as a Hub Controller for Axonemal Dynein Activities
Identification of the Outer-Inner Dynein Linker as a Hub Controller for Axonemal Dynein Activities
复制标题
DOI：
10.1016/j.cub.2013.03.028
复制
发表时间：
2013-04-22
期刊：
影响因子：
9.2
通讯作者：
Kikkawa, Masahide
中科院分区：
文献类型：
--
作者：
Oda, Toshiyuki;Yagi, Toshiki;Kikkawa, Masahide
背景：在鞭毛中，外动力蛋白臂（ODA）和内动力蛋白臂（IDA）在产生搏动运动中起着不同的作用。然而，两个动力蛋白之间的功能通信还没有调查。结果：在这里，我们证明了冷冻电子显微镜和化学交联的ODA的中间链2（IC 2）相互作用的动力蛋白调节复合物的轴丝，并构成的外-内动力蛋白（DYNA）连接器的一部分。此外，我们确定IC 2作为一个功能枢纽之间的ODA和IDA的基础上的表型衣原体突变体表达生物素标记的IC 2。的IC 2突变体的鞭毛显示激活微管滑动和增强ATP酶活性的ODA，以及改变的波形，表明衰减IDA activity.Conclusions：我们得出结论，该连接器控制ODA和IDAs和调节鞭毛跳动。
Background: In flagella, the outer dynein arm (ODA) and inner dynein arm (IDA) play distinct roles in generating beating motion. However, functional communications between the two dyneins have not been investigated.Results: Here, we demonstrated by cryo-electron microscopy and chemical crosslinking that intermediate chain 2 (IC2) of ODAs interacts with the dynein regulatory complex in the axoneme and constitutes part of the outer-inner dynein (OID) linker. Furthermore, we identified IC2 as a functional hub between ODAs and IDAs based on the phenotypes of Chlamydomonas mutants expressing biotinylation-tagged IC2. The flagella of the IC2 mutant showed activated microtubule sliding and enhanced ATPase activities of ODAs, as well as an altered waveform, indicating attenuated IDA activity.Conclusions: We concluded that the OID linker controls both ODAs and IDAs and regulates flagellar beating.