Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds
Acceleration of bone formation with BMP2 in frame-reinforced carbonate apatite-collagen sponge scaffolds
复制标题
DOI：
10.1007/s10047-007-0391-2
复制
发表时间：
2007-01-01
影响因子：
1.3
通讯作者：
Okazaki, Masayuki
中科院分区：
文献类型：
--
作者：
Hirata, Isao;Nomura, Yuji;Okazaki, Masayuki
除了具有高孔隙率和细胞容易侵入的大孔隙外，支架生物材料的发展还具有保持形状的特性。将合成的碳酸盐磷灰石（CO(3)Ap）与中和的胶原凝胶混合，在多孔羟基磷灰石（HAp）框架环中将CO(3)Ap-胶原混合物冻干成海绵。x射线衍射和傅里叶变换红外光谱（FT-IR）分析以及化学分析表明，合成的CO(3)Ap具有晶体性质，化学成分与骨头相似。扫描电镜（SEM）观察表明，CO(3) ap -胶原海绵具有适合细胞侵袭的细小孔隙。在成骨细胞的增殖和分化实验中，碱性磷酸酶和骨桥蛋白活性明显升高。将这些具有骨形态发生蛋白（rh-BMP2）的海绵框架复合物植入大鼠颅骨骨膜下，植入4周后，颅骨骨膜表面形成了明显的新骨。这些含有rh-BMP2的增强CO(3) ap -胶原海绵有望作为硬组织支架生物材料用于骨缺损的快速治愈。
The development is expected of scaffold biomaterials that feature a shape-maintaining property in addition to high porosity and large pores that cells can easily invade. To develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized carbonate apatite (CO(3)Ap) was mixed with neutralized collagen gel, and the CO(3)Ap-collagen mixtures were lyophilized into sponges in a porous hydroxyapatite (HAp) frame ring. X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR) analyses together with chemical analysis indicated that the synthesized CO(3)Ap had a crystalline nature and a chemical composition similar to that of bone. Scanning electron microscope (SEM) observation showed that the CO(3)Ap-collagen sponge had a sui pore size for cell invasion. In proliferation and differentiation experiments with osteoblasts, alkaline phosphatase and osteopontin activity were clearly detected. When these sponge-frame complexes with bone morphogenic protein (rh-BMP2) were implanted beneath the periosteurn cranii of rats, significant new bone was created at the surface of the periosteum cranii after 4 weeks of implantation. These reinforced CO(3)Ap-collagen sponges with rh-BMP2 are expected to be used as hard tissue scaffold biomaterials for the therapeutic purpose of the rapid cure of bone defects.