Germination response of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments
Germination response of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments
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DOI：
10.1111/j.1365-3180.2005.00477.x
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发表时间：
2005-12
期刊：
影响因子：
1.7
通讯作者：
W. Song;W. Zhou;Z. Jin;D. Cao;D. Joel;Y. Takeuchi;K. Yoneyama
中科院分区：
文献类型：
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作者：
W. Song;W. Zhou;Z. Jin;D. Cao;D. Joel;Y. Takeuchi;K. Yoneyama
雀麦(Orohanche spp.)是寄生杂草，会造成作物产量的重大损失。在不同温度、不同水势和不同植物生长调节剂的条件下，研究了人工发芽促进剂GR-24对3种Orobanche种子的影响。在18℃条件下处理7d，然后在18℃条件下进行萌发刺激，其发芽率最高。随着处理时间的延长(7、14、21和28天)，两种植物的发芽率逐渐降低。在-2 Mpa条件下，发芽率低于0和-1 Mpa条件下的发芽率，特别是在13℃和28℃条件下，Orohanche小种种子在18℃、23℃和28℃条件下仍能保持较高的发芽率，即使在显著延长的条件下(最长可达84天)。GA3(30~10 0 mg L-1)、去氟乐酮(10~10 0 mg L-1)和油菜素内酯(0.5~1.0 mg L-1)显著促进种子萌发，而0.0 1 mg L-1烯效唑显著降低3种罗班植物的发芽率。GA3和去甲氟拉松的促进作用和烯效唑的抑制作用，即使在治疗3d后也是明显的。在10~(-6)M直接暴露于GR24处理的罗班种子萌发明显降低，而GA3或去甲氟拉松(10~50 mg L~(-1))可减轻甚至消除GR24诱导的这种早期抑制作用。相反，烯效唑的加入增加了GR 24的抑制作用，尤其是对萝卜的抑制作用。
Broomrapes (Orohanche spp.) are parasitic weeds that cause significant losses of crop yield. Experiments were conducted to investigate the seed response to the artificial germination stimulant GR 24 in three species of Orobanche subjected to preconditioning under various temperatures, water potentials and with plant growth regulators. The highest germination percentages were observed in Orobanche ramosa, Orohanche aegyptiaca and Orohanche minor seeds conditioned at 18°C for 7 days followed by germination stimulation at 18°C. With the increase of the conditioning period (7, 14, 21 and 28 days), the germination percentage of O. ramosa and O. aegyptiaca progressively decreased. When conditioned at -2 MPa, the germination percentage was lower than at 0 and -1 MPa, especially at 13 and 28°C. Orohanche minor seeds could retain relatively high germination if conditioned at 18, 23 or 28°C, even after significantly extended conditioning periods (up to 84 days). GA 3 (30-100 mg L -1 ), norflurazon and fluridone (10-100 mg L -1 ), and brassinolide (0.5-1.0 mg L -1 ) increased seed germination, while 0.01 mg L -1 uniconazole significantly reduced germination rates of all three Orobanche spp. The promotional effects of GA 3 and norflurazon and the inhibitory effect of uniconazole were evident, even when they were treated for 3 days. Germination of Orobanche seeds was much lower when the unconditioned seeds were directly exposed to GR 24 at 10 -6 M. This early GR 24 -induced inhibition was however alleviated or even eliminated by the inclusion of GA 3 or norflurazon (10-50 mg L -1 ) in the conditioning medium. On the contrary, the inclusion of uniconazole increased the inhibitory effect of GR 24 , particularly in the case of O. ramosa.