Vru (Sub0144) controls expression of proven and putative virulence determinants and alters the ability of Streptococcus uberis to cause disease in dairy cattle.
Vru (Sub0144) controls expression of proven and putative virulence determinants and alters the ability of Streptococcus uberis to cause disease in dairy cattle.
复制标题
DOI：
10.1099/mic.0.055863-0
复制
发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Leigh JA
中科院分区：
文献类型：
--
作者：
Egan SA;Ward PN;Watson M;Field TR;Leigh JA
响应于不同环境刺激的基因表达的调节和控制对于病原体的成功适应和持久性至关重要。链球菌的调节基因vru编码与A组链球菌的Mga相似的独立反应调节因子。Mga控制许多重要的毒力决定因子的表达。奶牛乳房内接种突变型沙门氏菌的实验研究。与野生型菌株相比，在VRU中携带失活病变的菌株表现出降低的定殖乳腺的能力，并且不能诱导乳腺炎的临床症状。通过微阵列分析确定的突变体中基因表达的转录差异分析，鉴定了在Vru不存在下具有改变的表达的许多编码序列。这些由已知和推定的毒力决定因子组成，包括Lbp（Sub0145）、SclB（Sub1095）、PauA（Sub1785）和hasA（Sub1696）。
The regulation and control of gene expression in response to differing environmental stimuli is crucial for successful pathogen adaptation and persistence. The regulatory gene vru of Streptococcus uberis encodes a stand-alone response regulator with similarity to the Mga of group A Streptococcus. Mga controls expression of a number of important virulence determinants. Experimental intramammary challenge of dairy cattle with a mutant of S. uberis carrying an inactivating lesion in vru showed reduced ability to colonize the mammary gland and an inability to induce clinical signs of mastitis compared with the wild-type strain. Analysis of transcriptional differences of gene expression in the mutant, determined by microarray analysis, identified a number of coding sequences with altered expression in the absence of Vru. These consisted of known and putative virulence determinants, including Lbp (Sub0145), SclB (Sub1095), PauA (Sub1785) and hasA (Sub1696).
登录
查看更多内容
影响因子：
2.4
作者：
COLQUE, JIC;DEVRIESE, LA;HAESEBROUCK, F
通讯作者：
HAESEBROUCK, F
影响因子：
2.1
作者：
HILLERTON, JE;SHEARN, MFH;BOOTH, JM
通讯作者：
BOOTH, JM
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
2.1
作者：
BRAMLEY, AJ
通讯作者：
BRAMLEY, AJ
影响因子：
3.3
作者：
Leigh, JA;Hodgkinson, SM;Lincoln, RA
通讯作者：
Lincoln, RA