In situ Generation of RNA Complexes for Synthetic Molecular Strand-Displacement Circuits in Autonomous Systems
In situ Generation of RNA Complexes for Synthetic Molecular Strand-Displacement Circuits in Autonomous Systems
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DOI：
10.1021/acs.nanolett.0c03629
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发表时间：
2021-01-13
期刊：
影响因子：
10.8
通讯作者：
Ouldridge, Thomas E.
中科院分区：
文献类型：
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作者：
Bae, Wooli;Stan, Guy-Bart, V;Ouldridge, Thomas E.
实现DNA或RNA链置换反应的合成分子电路可用于构建复杂系统，如分子计算机和反馈控制系统。尽管最近取得了进展，但基于核酸的电路在体内的应用仍然具有挑战性，这是由于缺乏有效的方法来原位产生其基本组分，即称为门的多链复合物，即，在活细胞或其他自主系统中。在这里，我们提出了使用天然存在的自切割核酶切割单链RNA转录成一个门复合物的较短的链，从而打开了新的可能性，自主和连续生产的RNA链的化学计量和结构控制的方式。
Synthetic molecular circuits implementing DNA or RNA strand-displacement reactions can be used to build complex systems such as molecular computers and feedback control systems. Despite recent advances, application of nucleic acid-based circuits in vivo remains challenging due to a lack of efficient methods to produce their essential components, namely, multistranded complexes known as gates, in situ, i.e., in living cells or other autonomous systems. Here, we propose the use of naturally occurring self-cleaving ribozymes to cut a single-stranded RNA transcript into a gate complex of shorter strands, thereby opening new possibilities for the autonomous and continuous production of RNA strands in a stoichiometrically and structurally controlled way.