Quantification of microRNA expression with next-generation sequencing.
Quantification of microRNA expression with next-generation sequencing.
复制标题
DOI：
10.1002/0471142727.mb0417s103
复制
发表时间：
2013-07
影响因子：
--
通讯作者：
Seidman, J G
中科院分区：
文献类型：
--
作者：
Eminaga, Seda;Christodoulou, Danos C;Vigneault, Francois;Church, George M;Seidman, J G
下一代测序技术的快速发展使得全面有效地研究microRNAs(MiRNAs)的表达谱成为可能。我们之前已经证明，通过条形码对miRNA文库进行多路复用可以显著降低每个样本的测序成本，而不会影响文库质量。在本单元中，我们提供了一种分步分离miRNAs和构建多路miRNA文库的方法。我们还描述了一个定制的计算管道，设计用于分析基于Illumina的技术产生的多路复用miRNA文库测序读数。
Rapid advancement of next generation sequencing technologies has made it possible to study expression profiles of microRNAs (miRNAs) comprehensively and efficiently. We have previously shown that multiplexing miRNA libraries by barcoding can significantly reduce sequencing cost per sample without compromising library quality. In this unit, we provide a step-by-step protocol to isolate miRNAs and construct multiplexed miRNA libraries. We also describe a custom computational pipeline designed to analyze the multiplexed miRNA library sequencing reads generated by Illumina-based technology.