Frequencies of the separate human B cell subsets activatable to Ig secretion by Epstein-Barr virus and pokeweed mitogen
Frequencies of the separate human B cell subsets activatable to Ig secretion by Epstein-Barr virus and pokeweed mitogen
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EB 病毒和商陆有丝分裂原可激活 Ig 分泌的单独人类 B 细胞亚群的频率
DOI：
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发表时间：
1983
影响因子：
15.3
通讯作者：
S. Britton
中科院分区：
文献类型：
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作者：
O. Martínez;S. Britton
我们开发了一种微培养系统，适用于对 Epstein-Barr 病毒 (EBV) 和美洲商陆有丝分裂原 (PWM) 诱导的人类 B 细胞免疫球蛋白分泌激活进行有限稀释分析。研究发现，尽管需要填充 T 细胞来实现最佳 PWM 激活，但外源填充细胞不需要获得最佳 EBV 诱导的 B 细胞前体频率 (PF) 估计值。事实上，当使用自体 T 细胞作为填充细胞时，我们注意到 EBV 诱导的 IgM PF 显着下降。用环孢菌素 A 处理 T 细胞可以部分消除，而用 T 细胞照射可以完全消除这种减少。计算出的 EBV 激活的 B 细胞 IgM 的 PF 为 1/290 至 1/3,700，分泌 IgG 的 PF 为 1/920 至 1/3,250。 PWM激活1/140至1/3,200个B细胞分泌IgM。 EBV和PWM混合的实验结果表明，这两种多克隆激活剂作用于不同的B细胞亚群。因此，这两种药物似乎都能激活人外周血 B 细胞的小而离散的亚群以分泌 Ig。
We have developed a microculture system suitable for limiting dilution analysis of Epstein-Barr virus (EBV)- and pokeweed mitogen (PWM)- induced activation of immunoglobulin secretion by human B cells. It was found that exogenous filler cells were not required to obtain optimal EBV-induced B cell precursor frequency (PF) estimates, although filler T cells were required for optimal PWM activation. In fact, when autologous T cells were used as filler cells, a marked decrease in the EBV-induced IgM PF was noted. Treatment of the T cells with cyclosporin A partially eliminated, and irradiation of the T cells completely eliminated, this decrease. The calculated PF of B cells activated by EBV was from 1/290 to 1/3,700 for IgM, and from 1/920 to 1/3,250 for IgG secretion. PWM activated from 1/140 to 1/3,200 B cells to IgM secretion. The results of experiments in which EBV and PWM were mixed, indicated that these two polyclonal activators operated on different B cell subpopulations. Therefore, both these agents seem to activate small, discrete subpopulations of human peripheral blood B cells to Ig secretion.
小鼠红细胞形成的玫瑰花结定义了对美洲商陆有丝分裂原无反应的人类 B 淋巴细胞群体。
影响因子：
4.6
作者：
Lucivero,G;Lawton,AR;Cooper,MD
通讯作者：
Cooper,MD