Comparative Assessment of Anti-HLA Antibodies Using Two Commercially Available Luminex-Based Assays
Comparative Assessment of Anti-HLA Antibodies Using Two Commercially Available Luminex-Based Assays
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DOI：
10.1097/txd.0000000000000734
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发表时间：
2017-11-01
影响因子：
2.3
通讯作者：
Zorn, Emmanuel
中科院分区：
文献类型：
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作者：
Clerkin, Kevin J.;See, Sarah B.;Zorn, Emmanuel
背景同种异体抗HLA抗体（Abs）与实体器官移植物的排斥反应有关。Immucor公司和One Lambda/ThermoFisher公司生产了2种主要试剂盒，用于检测患者血清中的抗HLA抗体。我们试图比较两个平台的性能。方法.使用两个平台的背景调整平均荧光强度（MFI）值比较从125例移植前和移植后心脏和肺移植受者收集的血清。结果大多数HLA I类抗体（94.5%）和HLA II类抗体（89%）具有中等至高的MFI滴度（>= 4000）通过两种测定法检测。对于I类抗体（r = 0.3，r（2）= 0.09，P < 0.0001）和II类抗体（r = 0.707，r（2）= 0.5，P < 0.0001），从2种测定获得的MFI值之间观察到适度的相关性。两种试验均检测到另一种试验未检测到的抗I类和II类抗体;然而，两种试验均未优先检测到特异性HLA等位基因。对于有限数量的不一致血清，稀释导致2个平台之间的反应性特征相当。结论. Immucor和One Lambda/ ThermoFisher检测试剂盒具有相似但不相同的检测抗HLA Ab的能力。虽然测定之间存在相关性，但存在显著差异，其中一些可以通过稀释敏感的“前带”效应来解释。
Background. Allospecific anti-HLA antibodies (Abs) are associated with rejection of solid organ grafts. The 2main kits to detect anti-HLA Ab in patient serum are commercialized by Immucor and One Lambda/ThermoFisher. We sought to compare the performance of both platforms. Methods. Background-adjusted mean fluorescence intensity (MFI) values were used from both platforms to compare sera collected from 125 pretransplant and posttransplant heart and lung transplant recipients. Results. Most HLA class I (94.5%) and HLA class II (89%) Abs with moderate to high MFI titer (>= 4000) were detected by both assays. A modest correlation was observed between MFI values obtained from the 2 assays for both class I (r = 0.3, r(2) = 0.09, P < 0.0001) and class II Ab (r = 0.707, r(2) = 0.5, P < 0.0001). Both assays detected anti-class I and II Ab that the other did not; however, no specific HLA allele was detected preferentially by either of the 2 assays. For a limited number of discrepant sera, dilution resulted in comparable reactivity profiles between the 2 platforms. Conclusions. Immucor and One Lambda/ ThermoFisher assays have a similar, albeit nonidentical, ability to detect anti-HLA Ab. Although the correlation between the assays was present, significant variances exist, some of which can be explained by a dilution-sensitive "prozone" effect.