Satellite cells from dystrophic muscle retain regenerative capacity
Satellite cells from dystrophic muscle retain regenerative capacity
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DOI：
10.1016/j.scr.2014.10.007
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发表时间：
2015-01-01
影响因子：
1.2
通讯作者：
Morgan, Jennifer E.
中科院分区：
文献类型：
--
作者：
Boldrin, Luisa;Zammit, Peter S.;Morgan, Jennifer E.
杜氏肌营养不良症是一种遗传性疾病，其特征是进行性骨骼肌无力和萎缩，卫星细胞（肌肉干细胞）介导的肌肉维持/修复失败。营养不良性肌肉中的骨骼肌干细胞的功能可能会因处于日益增加的致病环境中以及不断需要修复肌肉而受到干扰。为了研究卫星细胞耗竭对此过程的影响，我们测试了从杜氏肌营养不良症 mdx 小鼠模型中分离出的卫星细胞的功能。我们发现，源自年轻 mdx 小鼠的卫星细胞有效地促进了我们体内小鼠模型中的肌肉再生。为了测试长期居住在营养不良环境中的影响，从老化的 mdx 肌肉中分离出卫星细胞。令人惊讶的是，它们的功能与来自年轻或老年野生型供体的那些一样。从营养不良的环境中去除卫星细胞表明，它们的再生能力保持完整，并且与来自健康肌肉的卫星细胞相似，这表明宿主环境对于控制卫星细胞功能至关重要。 (C) 2014 年作者。由 Elsevier B.V. 出版
Duchenne muscular dystrophy is an inherited disorder that is characterized by progressive skeletal muscle weakness and wasting, with a failure of muscle maintenance/repair mediated by satellite cells (muscle stem cells). The function of skeletal muscle stem cells resident in dystrophic muscle may be perturbed by being in an increasing pathogenic environment, coupled with constant demands for repairing muscle. To investigate the contribution of satellite cell exhaustion to this process, we tested the functionality of satellite cells isolated from the mdx mouse model of Duchenne muscular dystrophy. We found that satellite cells derived from young mdx mice contributed efficiently to muscle regeneration within our in vivo mouse model. To then test the effects of long-term residence in a dystrophic environment, satellite cells were isolated from aged mdx muscle. Surprisingly, they were as functional as those derived from young or aged wild type donors. Removing satellite cells from a dystrophic milieu reveals that their regenerative capacity remains both intact and similar to satellite cells derived from healthy muscle, indicating that the host environment is critical for controlling satellite cell function. (C) 2014 The Authors. Published by Elsevier B.V.