Structural and functional characterization of EIAV gp45 fusion peptide proximal region and asparagine-rich layer
Structural and functional characterization of EIAV gp45 fusion peptide proximal region and asparagine-rich layer
复制标题
EIAV gp45 融合肽近端区和富含天冬酰胺层的结构和功能表征
DOI：
10.1016/j.virol.2016.01.010
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发表时间：
2016-04-01
期刊：
影响因子：
3.7
通讯作者：
Liu, Xinqi
中科院分区：
文献类型：
--
作者：
Duan, Liangwei;Du, Jiansen;Liu, Xinqi
马传染性贫血病毒(EIAV)和人类免疫缺陷病毒(HIV)属于慢病毒属。与HIV gp41类似，EIAV gp45是一种融合蛋白，介导病毒颗粒和宿主细胞膜之间的融合。报道的gp45的晶体结构显示出与以前的HIV-1 gp41结构不同的构象，它包括融合肽近端区域(FPPR)和邻近的天冬酰胺富集层。包含一簇溶剂分子的复杂氢键网络似乎对gp45螺旋束的稳定性至关重要。有趣的是，病毒复制相对不受EIAV定点突变的影响，这与HIV-1的情况形成鲜明对比。基于这些观察，我们推测EIAV对新出现的突变具有更强的适应能力，这可能对EIAV作为准物种的进化具有重要意义，并可能有助于EIAV疫苗的成功。(C)2016 Elsevier Inc.保留所有权利。
Equine infectious anaemia virus (EIAV) and human immunodeficiency virus (HIV) are members of the lentiviral genus. Similar to HIV gp41, EIAV gp45 is a fusogenic protein that mediates fusion between the viral particle and the host cell membrane. The crystal structure of gp45 reported reveals a different conformation in the here that includes the fusion peptide proximal region (FPPR) and neighboring asparagine-rich layer compared with previous HIV-1 gp41 structures. A complicated hydrogen-bond network containing a cluster of solvent molecules appears to be critical for the stability of the gp45 helical bundle. Interestingly, viral replication was relatively unaffected by site-directed mutagenesis of EIAV, in striking contrast to that of HIV-1. Based on these observations, we speculate that EIAV is more adaptable to emergent mutations, which might be important for the evolution of EIAV as a quasi-species, and could potentially contribute to the success of the EIAV vaccine. (C) 2016 Elsevier Inc. All rights reserved.