ADHERENT BACTERIAL-COLONIZATION IN THE PATHOGENESIS OF OSTEOMYELITIS
ADHERENT BACTERIAL-COLONIZATION IN THE PATHOGENESIS OF OSTEOMYELITIS
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DOI：
10.1126/science.4001933
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
HOBGOOD, CD
中科院分区：
文献类型：
--
作者：
GRISTINA, AG;OGA, M;HOBGOOD, CD
骨髓炎骨手术清创过程中获得的材料的直接扫描电镜显示，感染细菌在附着的生物膜中以一致的微菌落生长，其范围如此之广，常常掩盖了受感染的骨表面。透射电镜显示该生物膜具有纤维基质，含有一些宿主细胞，并含有许多细菌，其周围通常集中有基质纤维。在这些生物膜中存在许多细菌形态，每个细菌都被胞外多糖聚合物包围，已知胞外多糖在自然生态系统和与生物材料相关的感染中介导微菌落的形成和细菌对表面的粘附。附着生长模式可能降低这些生物对宿主清除机制和抗生素治疗的敏感性，可能是[人类]急性和慢性骨髓炎的一个基本因素。
Direct scanning EM of material obtained during surgical debridement of osteomyelitic bone showed that the infecting bacteria grew in coherent microcolonies in an adherent biofilm so extensive it often obscured the infected bone surfaces. Transmission EM showed this biofilm to have a fibrous matrix, to contain some host cells, and to contain many bacteria around which matrix fibers were often concentrated. Many bacterial morphotypes were present in these biofilms and each bacterium was surrounded by exopolysaccharide polymers, which are known to mediate formation of microcolonies and adhesion of bacteria to surfaces in natural ecosystems and in infections related to biomaterials. The adherent mode of growth may reduce the susceptibility of these organisms to host clearance mechanisms and antibiotic therapy and may be a fundamental factor in acute and chronic osteomyelitis [in humans].