A micromethod for the determination of acid mucopolysaccharides in vascular tissue.
A micromethod for the determination of acid mucopolysaccharides in vascular tissue.
复制标题
一种测定血管组织中酸性粘多糖的微量方法。
DOI：
10.1016/s0021-9673(01)86097-0
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
I. Gore
中科院分区：
文献类型：
--
作者：
V. Stefanovich;I. Gore
由于不同大分子的结构相似性和尺寸差异（例如劳斯肉瘤中的 HA 的粒径比正常组织中的 HA 的粒径更小），人类和动物组织中不同酸性粘多糖 (AMPS*) l-* 的准确分离和定量测量变得复杂；由于从不同组织中分离出的单个 AMPS 的相对组成存在差异+，11，当尝试从仅含有少量这些物质的微小组织样本中定量分离和分级 AMPS 时，这些困难会被放大。KAPLAN 和 MEYER，I2 确定了人主动脉 AMPS 的成分为 HA、HS、ChS-I3（硫酸皮肤素、B-肝素）和 ChS-C（6-硫酸软骨素）。他们观察到四种 AMPS 的相对比例（尽管不是总量）随年龄的变化，但该技术需要大量样本，因此不适合测量单个主动脉的 AMPS 模式。其他人（例如 MANLEY~ 和 MANLEY AND KENT~）的微方法没有提供足够的信息来表明其分馏的定量有效性。同样的批评也适用于 TRUNDLE 和 MANN 的半微量（5 毫克）程序的报告，该程序是对 RINGERTZ 和 REICHARD 的修改~~，另外使用醋酸纤维素电泳，然后用吖啶橙染色后通过光密度测定进行定量。通过 ANTONOFOULOS 在 aZ 测定含有少至 z-5, ug 己糖胺的量。 1;但该方法相对复杂，并且没有区分硫酸软骨素组的成员。此外，在其应用于人类主动脉2时，只有通过牺牲定量回收率才能实现成分的完全分离。
Accurate isolation and quantitative measurement of the different acid mucopolysaccharides (AMPS*) l-* of human and animal tissues is complicated by the structural similarity of the different macromolecules, by variations in size (eg HA from Rous sarcoma has a smaller particle size than HA from normal tissueo); and by differences in the relative composition of individual AMPS isolated from different tissue+, 11, These difficulties are magnified when attempts are made to isolate and fractionate AMPS quantitatively from minute samples of tissue which contain only minor amounts of these substances.KAPLAN AND MEYER, I2 identified the constituents of human aortic AMPS as HA, HS, ChS-I3 (dermatan sulfate, B-heparin) and ChS-C (chondroitin 6-sulfate). They observed alterations in the relative proportions (though not the total amount) of the four AMPS with age, but this technique required large pooled samples, and, therefore, was not suitable for the measuring of the AMPS pattern of individual aortas. Micromethods by others, such as MANLEY~ and MANLEY AND KENT~ did not provide sufficient information to indicate the quantitative effectiveness of their fractionation. The same criticism can be applied to TRUNDLE AND MANN’@ report of a semi-micro (5 mg) procedure which is a modification of RINGERTZ AND REICHARD’S~~, using in addition electrophoresis on cellulose acetate followed by quantitation by densitometry after staining with acridine orange. Quantities containing as little as z-5, ug of hexosamine were assayed by ANTONOFOULOS at aZ. 1; but the method is relatively involved and does not distinguish members of the chondroitin sulfate group. Moreover, in its application to human aorta2 the complete separation of components was achieved only by sacrificing quantitative recovery.