Macrophage responses to silk
Macrophage responses to silk
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DOI：
10.1016/s0142-9612(03)00158-3
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发表时间：
2003-08-01
期刊：
影响因子：
14
通讯作者：
Kaplan, DL
中科院分区：
文献类型：
--
作者：
Panilaitis, B;Altman, GH;Kaplan, DL
丝纤维除了作为缝合线的传统用途之外，还具有潜在的生物医学应用。丝纤维和薄膜的物理特性使其成为组织工程支架应用的有希望的候选者，特别是在施加高机械负载或拉力的情况下或在需要低降解率的情况下。生物材料支架的一个关键问题是生物相容性。在体外系统中研究了完整丝纤维及其提取物的直接炎症潜力。 Tile 结果表明，丝纤维在 RAW 264.7 鼠巨噬细胞的短期和长期培养中很大程度上呈免疫惰性，而不溶性丝心蛋白颗粒则诱导显着的 TNF 释放。在天然丝纤维前提取的可溶性丝胶蛋白不会诱导显着的巨噬细胞活化。虽然丝胶本身不会激活巨噬细胞，但它与细菌脂多糖具有协同作用。丝纤维的低炎症潜力使其成为未来生物医学应用的有希望的候选者。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
Silk fibers have potential biomedical applications beyond their traditional use as Sutures. The physical properties of silk fibers and films make it a promising candidate for tissue engineering scaffold applications, particularly where high mechanical loads or tensile forces are applied or in cases where low rates of degradation Lire desirable. A critical issue for biomaterial scaffolds is biocompatibility. The direct inflammatory potential of intact silk fibers as well as extracts was studied in an in vitro system. Tile results indicate that silk fibers are largely immunologically inert in short- and long-term culture with RAW 264.7 murine macrophage cells while insoluble fibroin particles induced significant TNF release. Soluble sericin proteins extracted front native silk fibers did not induce significant macrophage activation. While sericin did not activate macrophages by itself, it demonstrated a synergistic effect with bacterial lipopolysaccharide. The low level of inflammatory potential of silk fibers makes them promising candidates in future biomedical applications. (C) 2003 Elsevier Science Ltd. All rights reserved.