Mass spectrometric imaging of peptide release from neuronal cells within microfluidic devices
Mass spectrometric imaging of peptide release from neuronal cells within microfluidic devices
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DOI：
10.1039/b706940e
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Sweedler, Jonathan V.
中科院分区：
文献类型：
--
作者：
Jo, Kyubong;Heien, Michael L.;Sweedler, Jonathan V.
微流体装置非常适合操作和测量质量有限的样品。在这里，我们采用包含功能化表面的微流体装置来化学刺激少量神经元（直至单个神经元），收集神经肽的释放，并使用质谱法对其进行表征。由于神经元中存在的肽只有一小部分在生理相关刺激下释放，因此可用于测量的材料量很小，因此需要最小的样品损失和高灵敏度检测。尽管许多检测方案都与微流体装置一起使用，但这里使用质谱检测，因为它的信息含量高，可以对释放的肽补体进行表征。不使用在线方法，而是使用离线分析；将肽收集到表面后，质谱成像会询问该表面以确定从细胞中释放的肽。该方案的整体实用性通过使用多种设备形式对加州海兔袋细胞神经元释放的神经肽进行测量来证明。
Microfluidic devices are well suited for manipulating and measuring mass limited samples. Here we adapt a microfluidic device containing functionalized surfaces to chemically stimulate a small number of neurons (down to a single neuron), collect the release of neuropeptides, and characterize them using mass spectrometry. As only a small fraction of the peptides present in a neuron are released with physiologically relevant stimulations, the amount of material available for measurement is small, thereby requiring minimal sample loss and high-sensitivity detection. Although a number of detection schemes are used with microfluidic devices, mass spectrometric detection is used here because of its high information content, allowing the characterization of the released peptide complement. Rather than using an on-line approach, off-line analysis is used; after collection of the peptides onto a surface, mass spectrometric imaging interrogates that surface to determine the peptides released from the cell. The overall utility of this scheme is demonstrated using several device formats with measurement of neuropeptides released from Aplysia californica bag cell neurons.