Multiple regulatory steps control histone mRNA concentrations.
Multiple regulatory steps control histone mRNA concentrations.
复制标题
多个调控步骤控制组蛋白 mRNA 浓度。
DOI：
10.1016/0968-0004(88)90027-8
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发表时间：
1988
影响因子：
13.8
通讯作者：
Pandey,NB
中科院分区：
文献类型：
--
作者：
Marzluff,WF;Pandey,NB
哺乳动物细胞中主要组蛋白的合成与DNA合成紧密耦合。组蛋白mRNA的浓度在细胞周期期间变化30-50倍，2并且mRNA浓度的变化与DNA合成的变化平行地非常迅速地发生。组蛋白mRNA浓度的降低通常发生在DNA合成后，可以通过用DNA合成抑制剂处理细胞来模拟3，4。我们实验室和其他研究哺乳动物细胞中组蛋白mRNA代谢控制的最近研究表明，组蛋白mRNA的代谢中存在多个控制步骤。主要的控制步骤是转录后，最近的研究已经开始了解这种新的控制mechardsm的分子基础。
The synthesis of the major histone proteins in mammalian cells is tightly coupled with DNA synthesis. The concentrations of the histone mRNAs vary 30-50-fold during the cell cycle, 2 and the changes in mRNA concentration occur very rapidly in parallel with changes in DNA synthesis. The decrease in histone mRNA concentrations which normally occurs following DNA synthesis can be mimicked by treating cells with inhibitors of DNA synthesis 3, 4. Recent studies in our laboratory and others studying control of histone mRNA metabolism in mammalian cells have revealed that there are multiple control steps in the metabolism of histone mRNA. The major control steps are post-transcriptional, and recent studies have led to a start towards understanding the molecular basis of this novel control mechardsm.