Attachment and detachment of cortical myosin regulates cell junction exchange during cell rearrangement in the Drosophila wing epithelium
Attachment and detachment of cortical myosin regulates cell junction exchange during cell rearrangement in the Drosophila wing epithelium
复制标题
皮质肌球蛋白的附着和脱离调节果蝇翼上皮细胞重排过程中的细胞连接交换
DOI：
10.1016/j.cub.2022.11.067
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发表时间：
2023
期刊：
影响因子：
9.2
通讯作者：
Sugimura Kaoru
中科院分区：
文献类型：
--
作者：
Ikawa Keisuke;Ishihara Shuji;Tamori Yoichiro;Sugimura Kaoru
上皮细胞重塑细胞粘附并改变它们的邻居以形成组织。这种细胞重排分三个步骤进行：连接处的收缩、连接处的交换和新产生的连接处的伸长。在此，通过结合实时成像和物理建模，我们表明，肌球蛋白-II（肌球蛋白-II）电缆周围的细胞顶点的形成下交换的交界处在theDrosophilawing上皮。肌II从细胞皮质的局部和短暂脱离受含LIM结构域的蛋白质Jub和三细胞分隔连接蛋白M6的调节。此外，我们发现M6转移到jubRNAi上的粘附连接平面，并且Jub在m6 RNAi细胞中持续保留在重新连接的连接处。Jub和M6之间的这种相互作用可以取决于连接长度，从而耦合皮质肌II电缆的分离和细胞重排期间连接的收缩/伸长。此外，我们开发了一个基于润湿理论的力学模型，并阐明了myo-II电缆的物理特性如何与结几何形状相结合，以诱导连接和分离状态之间的过渡，并支持细胞重排的单向性。总的来说，这项研究阐明了交换路口的几何，力学和信号的编排。
Epithelial cells remodel cell adhesion and change their neighbors to shape a tissue. This cellular rearrangement proceeds in three steps: the shrinkage of a junction, exchange of junctions, and elongation of the newly generated junction. Herein, by combining live imaging and physical modeling, we showed that the formation of myosin-II (myo-II) cables around the cell vertices underlies the exchange of junctions in theDrosophilawing epithelium. The local and transient detachment of myo-II from the cell cortex is regulated by the LIM domain-containing protein Jub and the tricellular septate junction protein M6. Moreover, we found that M6 shifts to the adherens junction plane onjubRNAi and that Jub is persistently retained at reconnecting junctions inm6RNAi cells. This interplay between Jub and M6 can depend on the junction length and thereby couples the detachment of cortical myo-II cables and the shrinkage/elongation of the junction during cell rearrangement. Furthermore, we developed a mechanical model based on the wetting theory and clarified how the physical properties of myo-II cables are integrated with the junction geometry to induce the transition between the attached and detached states and support the unidirectionality of cell rearrangement. Collectively, this study elucidates the orchestration of geometry, mechanics, and signaling for exchanging junctions.