Canalicular immunostaining of neprilysin (CD10) as a diagnostic marker for hepatocellular carcinomas
Canalicular immunostaining of neprilysin (CD10) as a diagnostic marker for hepatocellular carcinomas
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DOI：
10.1097/00000478-200110000-00011
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发表时间：
2001-10-01
影响因子：
5.6
通讯作者：
Röcken, C
中科院分区：
文献类型：
--
作者：
Borscheri, N;Roessner, A;Röcken, C
脑啡肽酶（CD 10）在正常和肿瘤性肝组织中均表达，其中它表现出与抗体与胆汁糖蛋白I（p-CEA）交叉反应时观察到的特征性小管模式（CD 10（can））相似的特征性小管模式。本回顾性研究的目的是探讨CD 10（can）在区分肝细胞癌（HCC; 63例标本）和转移至肝脏的非肝细胞癌（非HCC; 25例标本）中的应用。用抗CD 10、p-CEA和甲胎蛋白（AFP）的抗体进行免疫染色。用非放射性白蛋白原位杂交（ISH albumin）检测白蛋白mRNA。在HCC组中，43例（68.3%）标本中发现CD 10的小管染色模式，15例（23.8%）标本中发现AFP，60例（95.2%）标本中存在p-CEA的小管染色模式。35例HCC标本行ISH白蛋白检测，3例（85.7%）显示肿瘤细胞标记。在非HCC组中，CD 10（can）和p-CEA、AFP免疫染色和ISH白蛋白标记仅限于非肿瘤性肝组织。CD 10（can）、AFP、p-CEA和ISH白蛋白的敏感性和特异性分别为68.3%和100%、23.8%和100%、95.2%和100%、86.4%和100%。我们的研究结果表明，CD 10的小管染色是一个高度特异性的肝细胞分化的标志物。虽然它不能区分良性和恶性病变，但CD 10（can）显然有助于区分HCC和非HCC。
Neprilysin (CD 10) is expressed in both normal and neoplastic liver tissue, where it exhibits a characteristic canalicular pattern (CD10(can)) similar to the one observed when antibodies cross-react with biliary glycoprotein I (p-CEA). The aim of this retrospective study was to investigate the use of CD10(can) in differentiating between hepatocellular carcinomas (HCCs; 63 specimens) and nonhepatocellular carcinomas (non-HCCs) metastatic to the liver (non-HCC; 25 specimens). Immunostaining was performed with antibodies directed against CD10, p-CEA, and a-fetoprotein (AFP). Albumin mRNA was detected by nonradioactive in situ hybridization (ISHalbumin). In the HCC group a canalicular staining pattern for CD10 was found in 43 (68.3%) specimens, AFP was found in 15 (23.8%) specimens, and a canalicular staining pattern for p-CEA was present in 60 (95.2%) specimens. ISH albumin was performed in 35 HCC specimens and showed labeling of tumor cells in 3 (85.7%) specimens. In the non-HCC group CD10(can), and p-CEA, immunostaining for AFP and labeling for ISH albumin were confined to non-neoplastic liver tissue. Sensitivity and specificity were, respectively, 68.3% and 100% for CD10(can), 23.8% and 100% for AFP, 95.2% and 100% for canalicular p-CEA, and 86.4% and 100% for ISHalbumin. Our results demonstrate that canalicular staining for CD10 is a highly specific marker of hepatocytic differentiation. Although it does not differentiate between benign and malignant lesions, CD10(can) is clearly useful in differentiating between HCC and non-HCC.