Identification of key microRNAs affecting drip loss in porcine longissimus dorsi by RNA-Seq
Identification of key microRNAs affecting drip loss in porcine longissimus dorsi by RNA-Seq
复制标题
通过RNA-Seq鉴定影响猪背最长肌滴水损失的关键microRNA
DOI：
10.1016/j.gene.2018.01.005
复制
发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Wu Wangjun
中科院分区：
文献类型：
--
作者：
Wei Wei;Li Bojiang;Liu Kaiqing;Jiang Aiwen;Dong Chao;Jia Chao;Chen Jie;Liu Honglin;Wu Wangjun
MicroRNAs（miRNAs）是一类长度为22-nt的非编码小RNA，主要通过在转录后水平抑制蛋白质编码基因的表达，在多种代谢过程中发挥重要作用。滴水损失是影响猪肉品质的重要性状之一。到目前为止，DL性状的潜在调控因素仍不完全清楚。本研究构建了高DL（WJJ-H组）和低DL（WJJ-L组）个体背最长肌小RNA文库，并应用RNA-Seq技术筛选DL两极端表型个体差异表达的miRNAs。从WJJ-H和WJJ-L组中分别检测到184和176个猪已知的miRNAs。另外，两组间共鉴定出73个差异表达的miRNAs，其中40个上调，33个下调。此外，WJJ-H和WJJ-L组分别预测了133和140个新的潜在miRNA。值得注意的是，我们通过表达模式分析初步证实了miRNA-499和miRNA-22都是影响DL性状的潜在候选者。总之，本研究的结果丰富了对猪骨骼肌miRNAs的认识，为进一步阐明影响DL性状的miRNAs调控机制奠定了基础。
MicroRNAs (miRNAs) are a class of ~ 22-nt non-coding small RNA that play an important role in various metabolic processes, mainly through suppressing the expression of protein coding genes at post-transcriptional level. Drip loss (DL) is one of the most important meat quality traits affecting the end product yield and quality of pork. To date, the underlying regulatory factors involved in DL trait are still incompletely understood. In the present study, we constructed two small RNA libraries withlongissimus dorsimuscles from the higher DL (WJJ-H group) and the lower DL (WJJ-L group) individuals, and applied RNA-Seq technology to identify the differentially expressed miRNAs between the two extreme phenotypes of DL groups. A total of 184 and 176 porcine known miRNAs were detected from WJJ-H and WJJ-L groups, respectively. Moreover, 73 differentially expressed miRNAs were identified between two groups, of which 40 were up-regulated and 33 were down-regulated. In addition, 133 and 140 novel potential miRNAs were predicted from WJJ-H and WJJ-L groups, respectively. Notably, we preliminary confirmed that both miRNA-499 and miRNA-22 were potential candidates influencing DL trait by their expression pattern analysis. Overall, our data enhance the knowledge of porcine skeletal muscle miRNAs, and provide foundation for clarifying the miRNA regulatory mechanisms involved in DL trait.