Hematopoietic thymocyte precursors. I. Assay and kinetics of the appearance of progeny
Hematopoietic thymocyte precursors. I. Assay and kinetics of the appearance of progeny
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造血胸腺细胞前体细胞。
DOI：
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发表时间：
1976
影响因子：
15.3
通讯作者：
R. Basch
中科院分区：
文献类型：
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作者：
J. Kadish;R. Basch
建立了胸腺细胞造血前体细胞的定量分析方法。用此方法研究了经760 R照射的小鼠胸腺中骨髓和脾细胞输注后子代出现的动力学。前体细胞在骨髓中比在脾脏中常见七到八倍，并且在外周淋巴结中不存在。它们的数量随着动物的年龄而减少。当造血细胞在致命辐射后立即注射时，只有少量细胞实际上进入腺体。它们的后代在胸腺中8-12天内无法检测到。它们的检测时间取决于残留的内源性胸腺细胞群体的大小和注射的祖细胞的数量。已经提出的证据排除了胸腺损伤作为供体型细胞出现延迟的基础，并表明在受照射动物中产生“归巢”信号和前体细胞的发育都不是胸腺再增殖率的限制因素。这些研究表明，只需要极少量（少于100）的前胸腺细胞，以重建受辐射小鼠的胸腺。这种有限数量的祖细胞必须产生在再增殖后的第一周内观察到的T细胞免疫应答的整个库。
A quantitative assay for the hematopoietic precursor of thymocytes has been developed. Using this assay the kinetics of appearance of the progeny of transfused bone marrow and spleen cells in the thymus of irradiated (760 R) mice has been studied. Precursor cells are seven to eightfold more common in bone marrow than in spleen and are absent from peripheral lymph nodes. They decline in number as the animals age. When hematopoietic cells are injected immediately after lethal irradiation only a small number of cells actually enter the gland. Their progeny are not detectable in the thymus for 8-12 days. The time of their detection depends both upon the size of the residual endogenous thymocyte population and the number of progenitor cells injected. Evidence has been presented that excludes thymic injury as the basis for the delay in the appearance of donor type cells and indicates that neither the production of a "homing" signal in the irradiated animal nor the development of precursor cells are limiting factors in the rate of thymic repopulation. These studies indicate that only an exceedingly small number (less than 100) of prothymocytes are required to repopulate the thymus of an irradiated mouse. This restricted number of progenitors must produce the entire repertory of T-cell immunologic responsiveness seen in the first weeks after repopulation.