Discrete determinants in transfer RNA for editing and aminoacylation
Discrete determinants in transfer RNA for editing and aminoacylation
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DOI：
10.1126/science.276.5316.1250
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发表时间：
1997-05-23
期刊：
影响因子：
56.9
通讯作者：
Schimmel, P
中科院分区：
文献类型：
--
作者：
Hale, SP;Auld, DS;Schimmel, P
在翻译过程中，氨酰化错误在编辑反应中得到纠正，确保氨基酸稳定地附着到其相应的转移 RNA (tRNA) 上。先前的研究尚未表明实现翻译编辑所需的 tRNA 核苷酸与氨酰化所需的 tRNA 核苷酸是否相同或不同，但一些考虑表明它们是相同的。在这里，设计的 tRNA 对氨酰化具有高度活性，但对翻译编辑不具有活性。编辑反应可以通过操纵 L 形 tRNA 角上的核苷酸来控制。相反，这些操作不影响氨酰化。这些结果证明了 tRNA 编辑和氨酰化功能的核苷酸决定簇的分离。
During translation errors of aminoacylation are corrected in editing reactions which ensure that an amino acid is stably attached to its corresponding transfer RNA (tRNA). Previous studies have not shown whether the tRNA nucleotides needed for effecting translational editing are the same as or distinct from those required for aminoacylation, but several considerations have suggested that they are the same. Here, designed tRNAs that are highly active for aminoacylation but are not active in translational editing are presented. The editing reaction can be controlled by manipulation of nucleotides at the corner of the L-shaped tRNA. In contrast, these manipulations do not affect aminoacylation. These results demonstrate the segregation of nucleotide determinants for the editing and aminoacylation functions of tRNA.