Daily urinary excretion of bisphenol A.
Daily urinary excretion of bisphenol A.
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DOI：
10.1265/ehpm.9.22
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发表时间：
2004-01-01
影响因子：
4.7
通讯作者：
Shiraishi, Hiroaki
中科院分区：
文献类型：
--
作者：
Arakawa, Chikako;Fujimaki, Kayumi;Shiraishi, Hiroaki
目的：双酚A是一种内分泌干扰物，由于双酚A含有在通常用于保存食物和饮料的树脂和塑料中，因此引起了人们对从饮食中摄入双酚A的关注。本研究的目的是通过测量一组男性受试者的尿中总BPA来评估BPA的每日摄入水平（游离BPA加上用β-葡萄糖醛酸酶处理释放的BPA），以及确定BPA排泄的个体内变化。连续5天收集5名受试者的24小时尿液，用于评价-尿BPA排泄量的日变化和36名男性受试者的每日BPA摄入量水平的估计。BPA在尿样中的测定采用GC/MS/MS酶水解后的BPA葡萄糖醛酸，固相萃取，衍生化。结果：一个大的日间变化被发现超过5天的尿BPA的每日排泄量在5个主题。在36名男性受试者中，尿BPA的日排泄量呈对数正态分布，中位数为1.2 μ g/天（范围：
OBJECTIVES: Concerns over dietary exposure to bisphenol A (BPA), an endocrine disruptor, have been raised because BPA is contained in resins and plastics commonly used for the preservation of food and beverages. The purpose of the present study was to assess daily intake levels of BPA in a group of male subjects by measuring total urinary BPA (free BPA plus BPA released by treatment with beta-glucuronidase), as well as determining intra-individual variation in BPA excretion.METHODS: Twenty-four-hour urine was collected from 5 subjects for 5 consecutive days for the evaluation of between-day variation in urinary BPA excretion and from 36 male subjects for the estimation of the level of daily BPA intake. BPA in the urine samples was measured by GC/MS/MS following enzymatic hydrolysis of BPA glucuronate, solid phase extraction, and derivatization.RESULTS: A large between-day variation was found over 5 days for the daily excretion of urinary BPA in the 5 subjects. The daily excretion of urinary BPA was distributed log-normally in the 36 male subjects, with the median value being 1.2 mug/day (range: