Distribution of organ antigens over the surface of thyroid cells as examined by the immunofluorescence test.
Distribution of organ antigens over the surface of thyroid cells as examined by the immunofluorescence test.
复制标题
通过免疫荧光测试检查甲状腺细胞表面器官抗原的分布。
作者：
A. Fagraeus;J. Jonsson
使用含有甲状腺自身抗体的人血清和在猴中制备的抗HeLa细胞血清，通过间接免疫荧光技术检查悬浮的人甲状腺细胞表面上的器官抗原的分布。因此，后者的血清中含有种属抗体。发现人血清给出了一个点状的细胞轮廓，表明抗原间隔相当粗糙，而含有物种抗体的血清给出了一个完整的荧光细胞轮廓，表明抗原的一个相当连续的片。
The distribution of organ antigens over the surface of suspended human thyroid cells was examined by the indirect immunofluorescence technique using human sera containing thyroid autoantibodies and an anti-HeLa cell serum prepared in the monkey. The latter serum thus contained species antibodies. The human sera were found to give a dotted cell outline suggesting rather coarsely spaced antigens, while the serum containing species antibodies gave an unbroken fluorescent cell outline suggesting a rather continuous sheet of antigens.