Mice Stage-Specific Claudin 3 Expression Regulates Progression of Meiosis in Early Stage Spermatocytes
Mice Stage-Specific Claudin 3 Expression Regulates Progression of Meiosis in Early Stage Spermatocytes
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DOI：
10.1095/biolreprod.113.107847
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发表时间：
2013-07-01
影响因子：
3.6
通讯作者：
Kon, Yasuhiro
中科院分区：
文献类型：
--
作者：
Chihara, Masataka;Ikebuchi, Ryoyo;Kon, Yasuhiro
紧密连接蛋白3是紧密连接链的蛋白质组分。相邻支持细胞之间的紧密连接形成血睾屏障（BTB）。在精子发生过程中，生精阶段特异性表达的密蛋白3被认为是调节前细线期/细线期精母细胞穿过BTB的迁移。在这里，我们确定了在成年小鼠睾丸中表达密蛋白3的细胞类型，并研究了睾丸特异性体内敲低密蛋白3后的精子发生。原位杂交结果显示，claudin 3 mRNA主要表达在生殖细胞附近的曲细精管基板在VI-IX阶段。此外，claudin 3蛋白不仅定位于BTB，而且还定位于成年ICR睾丸中表达STRA 8的前细线期/细线期精母细胞的细胞膜。Cg-Tg（Stra 8-EGFP）1 Ysa/YsaRbrc小鼠。虽然claudin 3敲低不影响BTB的完整性，但它确实导致精母细胞迁移穿过BTB的部分延迟。此外，claudin 3敲低导致精子发生过程中的前细线期延长。这些数据表明，生精阶段特异性表达和定位的密蛋白3在精子发生过程中调节减数分裂的进程，通过促进生殖细胞迁移跨越BTB。
Claudin 3 is a protein component of the tight junction strands. Tight junctions between adjacent Sertoli cells form the blood-testis barrier (BTB). During spermatogenesis, seminiferous stage-specific expression of claudin 3 is believed to regulate the migration of preleptotene/leptotene spermatocytes across the BTB. Here, we determined the cell types expressing claudin 3 in adult mouse testis and investigated spermatogenesis after testis-specific in vivo knockdown of claudin 3. The results of in situ hybridization revealed that claudin 3 mRNA was predominantly expressed in germ cells near the basal lamina of seminiferous tubules at stages VI-IX. Furthermore, claudin 3 protein was localized not only to the BTB but also to the cell membrane of STRA8-expressing preleptotene/leptotene spermatocytes in the testis of adult ICR. Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc mice. Although claudin 3 knockdown did not affect BTB integrity, it did cause a partial delay in spermatocyte migration across the BTB. Moreover, claudin 3 knockdown resulted in a prolonged preleptotene phase during spermatogenesis. These data indicate that the seminiferous stage-specific expression and localization of claudin 3 during spermatogenesis regulate the progression of meiosis by promoting germ cell migration across the BTB.