Protective mechanism of gallic acid and its novel derivative against ethanol-induced gastric ulcerogenesis: Involvement of immunomodulation markers, Hsp70 and Bcl-2-associated X protein
Protective mechanism of gallic acid and its novel derivative against ethanol-induced gastric ulcerogenesis: Involvement of immunomodulation markers, Hsp70 and Bcl-2-associated X protein
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DOI：
10.1016/j.intimp.2013.04.005
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发表时间：
2013-06-01
影响因子：
5.6
通讯作者：
Abdelwahab, Siddig Ibrahim
中科院分区：
文献类型：
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作者：
Abdelwahab, Siddig Ibrahim
一些植物化学物质通过抑制胃酸分泌或通过抗氧化作用表现出胃保护作用。这些抗氧化植物化学物质之一，已被研究是没食子酸。然而，其治疗和预防胃溃疡的机制尚不清楚。本研究旨在探讨没食子酸（GA）及其新型合成衍生物（GD）的抗溃疡作用机制。成年雄性Sprague道利大鼠经口给予GA和GD预处理，30 min后暴露于95%乙醇（5 ml/kg）诱导的急性胃溃疡形成。通过定性和定量评价胃病变、胃液酸度、粘液产生、组织病理学、PAS组织化学、Hsp 70和Bax免疫染色、一氧化氮、前列腺素E-2、TNF-α和硫代巴比妥酸反应物质（TBARS）测定来评估GA和GD的潜在胃化学预防作用。口服GA和GD（25和50 mg/kg）可显著抑制乙醇引起的胃粘膜损伤（P < 0.05）。这些化合物对大鼠胃有保护作用，并显著调节PAS反应物质、胃pH、TBARS、免疫和凋亡标志物的水平。没食子酸（GA）及其新衍生物（GD）的胃保护活性可能与其体内抗氧化特性、免疫调节蛋白和抑制线粒体凋亡有关。(c)2013爱思唯尔有限公司版权所有。
Some phytochemicals demonstrate gastroprotective effects by inhibiting gastric acid secretion or through antioxidant action. One of these antioxidant phytochemicals which have been studied is gallic acid. However, its mechanism in the treatment and prevention of gastric ulcer remains unclear. This study evaluated the anti-ulcerogenic mechanism(s) of gallic acid (GA) and its novel synthetic derivative (GD). Adult male Sprague Dawley rats were orally pretreated with GA and GD and 30 min later exposed to acute gastric ulcerogenesis induced by 95% ethanol (5 ml/kg). Potential gastric chemoprevention of GA and GD were assessed using qualitative and quantitative evaluation of gastric lesions, gastric juice acidity, mucus production, histolopathology, PAS histochemistry, immunostaining of Hsp70 and Bax, nitric oxide, prostaglandin E-2, TNF-alpha and thiobarbituric acid reactive substances (TBARS) assay. Oral administration of GA and GD (25 and 50 mg/kg) inhibited significantly (P < 0.05) ethanol-induced gastric lesions. Treatment with the compounds protected rat's stomach and modulated remarkably the levels of PAS-reactive substances, gastric pH, TBARS, immunological and apoptosis marker. The in vivo antioxidant properties, immunomodulator proteins and inhibition of mitochondrial apoptosis may contribute to the gastroprotective activity of gallic acid (GA) and its novel derivative (GD). (c) 2013 Elsevier B.V. All rights reserved.