Frog Virus 3 dissemination in the brain of tadpoles, but not in adult Xenopus, involves blood brain barrier dysfunction.
Frog Virus 3 dissemination in the brain of tadpoles, but not in adult Xenopus, involves blood brain barrier dysfunction.
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DOI：
10.1038/srep22508
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发表时间：
2016-03-02
影响因子：
4.6
通讯作者：
Robert J
中科院分区：
文献类型：
--
作者：
De Jesús Andino F;Jones L;Maggirwar SB;Robert J
虽然越来越多的证据表明单核细胞在两栖动物宿主防御中起着关键作用，但单核细胞也被认为在由蛙病毒引起的传播和持续感染中起重要作用。然而，人们对受感染的巨噬细胞的命运知之甚少，也不知道蛙病毒是否利用免疫特权器官（如大脑）来建立储库。建立了两栖动物非洲爪蟾和蛙病毒3型（FV3）的动物模型，作为研究蛙病毒是否能在体内传播到脑的实验平台。我们的数据表明，FV3感染改变了血脑屏障的完整性，可能是由炎症反应介导的，这导致病毒传播到X的中枢神经系统。leevis蝌蚪，但不是成年。此外，我们的数据表明，巨噬细胞通过携带病毒进入神经组织在病毒传播中发挥重要作用。
While increasing evidence points to a key role of monocytes in amphibian host defenses, monocytes are also thought to be important in the dissemination and persistent infection caused by ranavirus. However, little is known about the fate of infected macrophages or if ranavirus exploits immune privileged organs, such as the brain, in order to establish a reservoir. The amphibian Xenopus laevis and Frog Virus 3 (FV3) were established as an experimental platform for investigating in vivo whether ranavirus could disseminate to the brain. Our data show that the FV3 infection alters the BBB integrity, possibly mediated by an inflammatory response, which leads to viral dissemination into the central nervous system in X. laevis tadpole but not adult. Furthermore, our data suggest that the macrophages play a major role in viral dissemination by carrying the virus into the neural tissues.