Successful serial imaging of the mouse cerebral arteries using conventional 3-T magnetic resonance imaging.
Successful serial imaging of the mouse cerebral arteries using conventional 3-T magnetic resonance imaging.
复制标题
使用传统 3-T 磁共振成像成功对小鼠脑动脉进行连续成像。
DOI：
10.1038/jcbfm.2015.78
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Umemura,Kazuo
中科院分区：
文献类型：
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作者：
Makino,Hiroshi;Hokamura,Kazuya;Natsume,Takahiro;Kimura,Tetsuro;Kamio,Yoshinobu;Magata,Yasuhiro;Namba,Hiroki;Katoh,Takasumi;Sato,Shigehito;Hashimoto,Tomoki;Umemura,Kazuo
系列影像学研究可用于表征各种小鼠脑动脉的病理和生理性重塑。我们测试了使用现成的常规3-T磁共振成像（MRI）连续成像小鼠脑血管重构的可行性。我们利用小鼠颅内动脉瘤模型作为小鼠脑动脉动态病理性重塑模型。动脉瘤是由高血压和单一弹性蛋白酶注入脑脊液引起的。对于小鼠脑血管成像，我们使用传统的3-T MRI系统和40 mm鞍形线圈。我们采用非增强磁共振血管造影（MRA）检测颅内动脉瘤形成，t2加权成像检测动脉瘤性蛛网膜下腔出血。每2至3天进行一次连续MRI检查。动脉瘤形成和蛛网膜下腔出血的MRI检测与死后脑灌注染色检查进行比较。MRA和t2加权成像时间分别为3.7±0.5分钟和4.8±0.0分钟。所有动脉瘤和蛛网膜下腔出血均由两名蒙面观察者在MRI上正确识别。这种基于mri的连续成像技术在检测小鼠颅内动脉瘤形成和蛛网膜下腔出血方面是有用的。
Serial imaging studies can be useful in characterizing the pathologic and physiologic remodeling of cerebral arteries in various mouse models. We tested the feasibility of using a readily available, conventional 3-T magnetic resonance imaging (MRI) to serially image cerebrovascular remodeling in mice. We utilized a mouse model of intracranial aneurysm as a mouse model of the dynamic, pathologic remodeling of cerebral arteries. Aneurysms were induced by hypertension and a single elastase injection into the cerebrospinal fluid. For the mouse cerebrovascular imaging, we used a conventional 3-T MRI system and a 40-mm saddle coil. We used non-enhanced magnetic resonance angiography (MRA) to detect intracranial aneurysm formation and T2-weighted imaging to detect aneurysmal subarachnoid hemorrhage. A serial MRI was conducted every 2 to 3 days. MRI detection of aneurysm formation and subarachnoid hemorrhage was compared against the postmortem inspection of the brain that was perfused with dye. The imaging times for the MRA and T2-weighted imaging were 3.7 ± 0.5 minutes and 4.8 ± 0.0 minutes, respectively. All aneurysms and subarachnoid hemorrhages were correctly identified by two masked observers on MRI. This MRI-based serial imaging technique was useful in detecting intracranial aneurysm formation and subarachnoid hemorrhage in mice.