RETINOIC ACID IS ENRICHED IN HENSEN NODE AND IS DEVELOPMENTALLY REGULATED IN THE EARLY CHICKEN-EMBRYO
RETINOIC ACID IS ENRICHED IN HENSEN NODE AND IS DEVELOPMENTALLY REGULATED IN THE EARLY CHICKEN-EMBRYO
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DOI：
10.1073/pnas.89.21.10056
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发表时间：
1992-11-01
影响因子：
11.1
通讯作者：
SOLURSH, M
中科院分区：
文献类型：
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作者：
CHEN, YP;HUANG, L;SOLURSH, M
视黄酸（RA）被认为是鸡肢中潜在的形态原，也被认为参与早期胚胎发育。生物活性的基础上，以前的报告表明，亨森的节点，在鸡的Spemann的组织者的解剖等价物，可能含有RA。在这里，通过使用分子检测系统，我们证明，亨森结含有维甲酸的浓度几乎等于20倍以上，在邻近组织。此外，第6阶段的亨森氏结含有几乎等于3倍以上的视色素比第4阶段的胚胎。这些内源性类维生素A可以建立从亨森结到邻近组织的浓度梯度，并在脊椎动物中建立初级胚胎轴中发挥作用。结果还表明，类维生素A的浓度在亨森结发育调节。
Retinoic acid (RA) has been considered as a potential morphogen in the chicken limb and has also been suggested to be involved in early embryonic development. On the basis of biological activity, previous reports suggest that Hensen's node, the anatomical equivalent in the chicken of the Spemann's organizer, may contain RA. Here, by using a molecular assay system, we demonstrate that Hensen's node contains retinoids in a concentration almost-equal-to 20 times more than that in the neighboring tissues. Furthermore, stage 6 Hensen's node contains almost-equal-to 3 times more retinoid than that of stage 4 embryos. These endogenous retinoids may establish a concentration gradient from Hensen's node to adjacent tissues and play a role in establishing the primary embryonic axis in the vertebrate. The results also suggest that the retinoid concentration in Hensen's node is developmentally regulated.