Brassinosteroids induce plant tolerance against phenanthrene by enhancing degradation and detoxification in Solanum lycopersicum L.
Brassinosteroids induce plant tolerance against phenanthrene by enhancing degradation and detoxification in Solanum lycopersicum L.
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油菜素类固醇通过增强番茄的降解和解毒来诱导植物对菲的耐受性。
DOI：
10.1016/j.ecoenv.2012.02.004
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发表时间：
2012-06-01
影响因子：
6.8
通讯作者：
Yu, Jing-Quan
中科院分区：
文献类型：
--
作者：
Ahammed, Golam Jalal;Gao, Chun-Juan;Yu, Jing-Quan
多环芳烃（PAHs）对植物和动物都是有毒的。提高植物的耐受性和解毒能力对植物修复多环芳烃具有重要意义。因此，我们研究了24-表油菜素内酯（EBR）对番茄（Solanum lycopersicum L.）植株三环多环芳烃（菲- phe）代谢和随后的抗逆性的影响。暴露于PHE (300 μ M) 21 d显著降低了生物量和净CO2同化（P-n），但诱导了光抑制、丙二醛（MDA）、H2O2和抗氧化酶。ph处理后的根尖细胞超微结构发生明显改变。叶面施用EBR （0.1 μ M）显著提高了生物量。P-n和抗氧化酶活性，但MDA和H2O2降低，使根细胞免受严重损伤。EBR+PHE处理植株的解毒基因（CYP90b3、GSH1、GST1）表达、还原性谷胱甘肽（GSH）含量和谷胱甘肽s -转移酶活性均高于单独处理植株。此外，由于EBR+PHE处理，观察到根部的PHE残留物水平较低。综上所述，我们的结果强烈表明EBR对PHE的解毒和降解有增强和协调的作用。(C) 2012爱思唯尔公司版权所有。
Polycyclic aromatic hydrocarbons (PAHs) are toxic to both plants and animals. The enhancement of plant tolerance and detoxification capacity is important for the plant-based remediation of PAHs. Therefore, we investigated the effects of 24-epibrassinolide (EBR) on the metabolism of a three-ringed PAH (phenanthrene-PHE) and subsequent stress tolerance in tomato (Solanum lycopersicum L.) plants. Exposure to PHE (300 mu M) for 21 d significantly decreased biomass and net CO2 assimilation (P-n) but induced photoinhibition, malondialdehyde (MDA), H2O2 and antioxidant enzymes. Obvious ultrastructural alterations were observed in the PHE-treated root tip cells. Importantly, the foliar application of EBR (0.1 mu M) significantly increased biomass. P-n and antioxidant enzyme activities but decreased MDA and H2O2 compared with PHE alone and saved the root cells from severe damage. The expression of detoxification genes (CYP90b3, GSH1, GST1), reduced glutathione (GSH) content and glutathione S-transferase activity in the EBR+PHE-treated plants were higher than those of PHE alone. Additionally, lower levels of PHE residues in the roots were observed as a result of EBR+PHE treatment. Taken together, our results strongly suggest an enhanced and coordinated detoxification and degradation of PHE by EBR. (C) 2012 Elsevier Inc. All rights reserved.