Catabolism of L-arginine by Pseudomonas aeruginosa.
Catabolism of L-arginine by Pseudomonas aeruginosa.
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铜绿假单胞菌对 L-精氨酸的分解代谢。
DOI：
10.1099/00221287-116-2-381
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
V. Stalon
中科院分区：
文献类型：
--
作者：
A. Mercenier;J. Simon;D. Haas;V. Stalon
已知铜绿假单胞菌通过精氨酸脱亚胺酶途径分解精氨酸。现在已经发现了另一种途径，其中精氨酸以胍丁胺和N-氨基甲酰腐胺作为中间体转化为腐胺。在粗提物中检测到属于该途径的以下酶活性：精氨酸脱羧酶（EC 4.1.1.19），催化精氨酸释放二氧化碳以产生胍丁胺；胍丁胺脱亚胺酶（EC 3.5.3.12），其将胍丁胺降解为 N-氨基甲酰腐胺；和N-氨基甲酰腐胺脒基水解酶(EC 3.5.3.-)，然后去除氨基甲酰腐胺的脲基。在粗提取物中，精氨酸脱羧酶活性受到磷酸吡哆醛、Mg2+ 以及分解代谢途径的产物腐胺和亚精胺的刺激。以精氨酸作为唯一碳源和氮源的铜绿假单胞菌的生长显着增加了精氨酸脱羧酶的活性。胍丁胺和N-氨基甲酰腐胺诱导胍丁胺脱亚胺酶和N-氨基甲酰腐胺水解酶的合成。在含有精氨酸或胍丁胺的培养基中添加琥珀酸盐或柠檬酸盐会抑制精氨酸脱羧酶途径中涉及的酶。此外，当铜绿假单胞菌在含有胍丁胺加谷氨酰胺或胍丁胺加琥珀酸盐和氨的培养基中生长时，对胍丁胺脱亚胺酶和N-氨基甲酰腐胺水解酶的抑制进一步增强。这表明胍丁胺途径的表达可能受到碳分解代谢物抑制和氮分解代谢物抑制的调节。
Pseudomonas aeruginosa is known to break down arginine by the arginine deiminase pathway. An additional pathway has now been found whereby arginine is converted to putrescine with agmatine and N-carbamoylputrescine as intermediates. The following enzyme activities belonging to this pathway were detected in crude extracts: arginine decarboxylase (EC 4.1.1.19), which catalyses the release of CO2 from arginine to give agmatine; agmatine deiminase (EC 3.5.3.12), which degrades agmatine to N-carbamoylputrescine; and N-carbamoylputrescine amidinohydrolase (EC 3.5.3.-), which then removes the ureido group of carbamoylputrescine. In crude extracts, arginine decarboxylase activity was stimulated by pyridoxal phosphate, Mg2+ and by the products of the catabolic pathway, putrescine and spermidine. Growth of P. aeruginosa on arginine as the sole carbon and nitrogen source markedly increased the activity of arginine decarboxylase. Agmatine and N-carbamoylputrescine induced the synthesis of agmatine deiminase and N-carbamoylputrescine hydrolase. Addition of succinate or citrate to medium containing arginine or agmatine led to repression of the enzymes involved in the arginine decarboxylase pathway. Moreover, the repression of agmatine deiminase and N-carbamoylputrescine hydrolase was further increased when P. aeruginosa was grown in media with agmatine plus glutamine or agmatine plus succinate and ammonia. This suggests that the expression of the agmatine pathway may be regulated by carbon catabolite repression as well as nitrogen catabolite repression.