Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes
Purification and assay of the human INO80 and SRCAP chromatin remodeling complexes
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DOI：
10.1016/j.ymeth.2006.06.023
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发表时间：
2006-12-01
期刊：
影响因子：
4.8
通讯作者：
Conaway, Ronald C.
中科院分区：
文献类型：
--
作者：
Cai, Yong;Jin, Jingji;Conaway, Ronald C.
染色质修饰和重塑酶在染色体生物学的转录、复制、重组和修复等方面起着至关重要的作用。我们的实验室最近鉴定并表征了两种多亚基人类染色质重塑酶，称为INO80和SRCAP复合物。机制研究表明，人INO80复合物催化核小体滑动，SRCAP复合物催化含有组蛋白变体H2A的组蛋白H2A/H2B二聚体的atp依赖性交换。进入核小体。在这里，我们描述了INO80和SRCAP染色质重塑复合物的纯化和测定方法。(c) 2006爱思唯尔公司版权所有。
Chromatin modifying and remodeling enzymes play critical roles in many aspects of chromosome biology including transcription, replication, recombination, and repair. Our laboratory recently identified and characterized two multisubunit human chromatin remodeling enzymes designated the INO80 and SRCAP complexes. Mechanistic studies revealed that the human INO80 complex catalyzes nucleosome sliding and the SRCAP complex catalyzes ATP-dependent exchange of histone H2A/H2B dimers containing the histone variant H2A.Z into nucleosomes. Here we describe methods for purification and assay of the INO80 and SRCAP chromatin remodeling complexes. (c) 2006 Elsevier Inc. All rights reserved.