Efficient NES-dependent protein nuclear export requires ongoing synthesis and export of mRNAs.
Efficient NES-dependent protein nuclear export requires ongoing synthesis and export of mRNAs.
复制标题
有效的 NES 依赖性蛋白质核输出需要 mRNA 的持续合成和输出。
DOI：
10.1016/j.yexcr.2004.03.051
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ljungman,Mats
中科院分区：
文献类型：
--
作者：
O'Hagan,HeatherM;Ljungman,Mats
调节蛋白质核输出的机制尚不完全清楚。为了研究蛋白质核输出的效率是否取决于正在进行的RNA合成和/或mRNA核输出，我们使用了含有核输出信号（NES）的荧光报告蛋白的显微注射方法，并在mRNA合成或输出被抑制的条件下对人成纤维细胞的蛋白质输出进行了评分。我们发现转录抑制显著减弱了nes依赖的核输出。此外，通过共显微注射RNAse A来消化内源性核rna可以抑制nes依赖性核输出。最后，在通过微量注射抗tap抗体或通过表达显性阴性形式的NUP160特异性阻断mrna核输出的细胞中，NES报告蛋白的核输出被显著抑制。这些结果表明，mrna的合成和输出在nes依赖性蛋白核输出中具有新的作用。
The mechanisms regulating nuclear export of proteins are not fully understood. To investigate whether the efficiency of protein nuclear export may depend on ongoing RNA synthesis and/or mRNA nuclear export, we used a microinjection approach with a fluorescent reporter protein containing a nuclear export signal (NES) and scored protein export in human fibroblasts under conditions when the synthesis or export of mRNAs was inhibited. We show that inhibition of transcription significantly attenuated generic NES-dependent nuclear export. Furthermore, digestion of endogenous nuclear RNAs by co-microinjection of RNAse A inhibited NES-dependent nuclear export. Finally, nuclear export of the NES reporter protein was significantly inhibited in cells in which nuclear export of mRNAs had been specifically blocked by microinjection of anti-TAP antibodies or by expression of a dominant negative form of NUP160. These results demonstrate a novel role for ongoing synthesis and export of mRNAs in NES-dependent protein nuclear export.