Frequency of secondary symbiont infection in an invasive psyllid relates to parasitism pressure on a geographic scale in California
Frequency of secondary symbiont infection in an invasive psyllid relates to parasitism pressure on a geographic scale in California
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DOI：
10.1128/aem.01672-07
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发表时间：
2007-12-01
影响因子：
4.4
通讯作者：
Stouthamer, R.
中科院分区：
文献类型：
--
作者：
Hansen, A. K.;Jeong, G.;Stouthamer, R.
两个内共生体，一个专性初级共生体和一个兼性次生共生体，在入侵的红桉树（桉树）内，Glycaspis brimleecombei。利用PCR检测、限制性内切酶、克隆和测序等方法，对加州多个种群中G. brimblecombei次生共生体的多样性和频率进行了广泛调查。对19个种群的380个黄颡鱼个体进行了次生共生体筛选。分子筛选结果表明，在加利福尼亚地区的绿腹小檗种群中只存在一种次生共生体。总体而言，筛选的380只木虱中有40%感染了次生共生体。有趣的是，木虱种群的次生共生体感染频率在0 ~ 75%之间变化显著，且与木虱的寄生压力显著相关。
Two endosymbionts, an obligate primary symbiont and a facultative secondary symbiont, are harbored within the invasive red gum (eucalyptus) lerp psyllid, Glycaspis brimblecombei, in California. An extensive survey of diversity and frequency of G. brimblecombei's secondary symbiont in multiple populations throughout the state of California was conducted using PCR detection, restriction enzymes, cloning, and sequencing. A total of 380 G.brimblecombei individuals in 19 populations were screened for secondary symbionts. Based on molecular screening results, only one type of secondary symbiont was present in G. brimblecombei populations in California. Overall, 40% of the 380 psyllids screened were infected with the secondary symbiont. Interestingly, secondary symbiont infection frequencies in G. brimblecombei populations varied dramatically from 0 to 75% and were significantly related to parasitism pressure by Psyllaphaegus bliteus, a solitary endoparasitoid of the psyllid.