Regulatory function of the novel transcription factor CxrC in Penicillium oxalicum
Regulatory function of the novel transcription factor CxrC in Penicillium oxalicum
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草酸青霉中新型转录因子 CxrC 的调节功能。
DOI：
10.1111/mmi.14843
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发表时间：
2021-11-30
影响因子：
3.6
通讯作者：
Zhao, Shuai
中科院分区：
文献类型：
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作者：
Zhang, Ting;Mai, Rong-Ming;Zhao, Shuai
子囊菌真菌中的许多转录因子（TFs）在细胞过程中起着至关重要的作用；然而，人们对它们的功能知之甚少。在这里，我们鉴定并表征了一个新的TF CxrC (POX01387)，它作用于关键TF CxrA的下游，这是草青霉生产植物生物质降解酶（PBDE）所必需的。草藻中cxrC的缺失显著影响了多溴二苯醚的产生，以及菌丝生长和分生孢子的产生。转换到Avicel培养约12小时后，CxrA直接抑制cxrC的表达。CxrC结合了PBDE主要基因和分生孢子发育相关基因的启动子。发现CxrC结合了TSSGTYR核心序列（S: C和G； Y: T和C； R: G和A）的重要纤维素酶基因cbh1和eg1。研究发现，CxrC的N端肽和c端肽以及CxrC的磷酸化均介导其二聚体化。CxrC中的保守基序LPSVRSLLTP（65-74）被发现是调节纤维素酶生产所必需的。本研究揭示了子囊菌真菌中tnf介导的多溴二苯醚基因和参与细胞过程的基因表达调控的新机制。
Numerous transcription factors (TFs) in ascomycete fungi play crucial roles in cellular processes; however, how most of them function is poorly understood. Here, we identified and characterized a novel TF, CxrC (POX01387), acting downstream of the key TF CxrA, which is essential for plant-biomass-degrading-enzyme (PBDE) production in Penicillium oxalicum. Deletion of cxrC in P. oxalicum significantly affected the production of PBDEs, as well as mycelial growth and conidiospore production. CxrA directly repressed the expression of cxrC after about 12 hr following switch to Avicel culture. CxrC bound the promoters of major PBDE genes and genes involved in conidiospore development. CxrC was found to bind the TSSGTYR core sequence (S: C and G; Y: T and C; R: G and A) of the important cellulase genes cbh1 and eg1. Both N- and C-terminal peptides of CxrC and the CxrC phosphorylation were found to mediate its homodimerization. The conserved motif LPSVRSLLTP (65-74) in CxrC was found to be required for regulating cellulase production. This study reveals novel mechanisms of TF-mediated regulation of the expression of PBDE genes and genes involved in cellular processes in an ascomycete fungus.