Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region
Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region
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DOI：
10.1128/jcm.43.12.6176-6182.2005
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发表时间：
2005-12-01
影响因子：
9.4
通讯作者：
Palacios, G
中科院分区：
文献类型：
--
作者：
Casas, I;Avellon, A;Palacios, G
在这里，我们提出了一种基于保守六邻体基因片段的 PCR 扩增和系统发育分析的腺病毒检测和基因分型系统。该系统使用 157 个序列（86 个先前分型的样本和 71 个临床样本）进行了验证，并分别正确识别了 100% 和 84% 的序列的物种和血清型。特定血清型和临床综合征之间的已知关联已得到验证。描述了可能的新关联以允许进一步的独立测试。
Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.