Studies on the intracellular synthesis of reovirus-specified proteins.
Studies on the intracellular synthesis of reovirus-specified proteins.
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DOI：
10.1016/0042-6822(70)90171-6
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发表时间：
1970-07
期刊：
影响因子：
3.7
通讯作者：
H. Zweerink;W. Joklik
中科院分区：
文献类型：
--
作者：
H. Zweerink;W. Joklik
所有呼肠孤病毒衣壳多肽在小鼠L成纤维细胞中以可测量的量形成。其中五个（λ1、λ2、μ1、σ2和σ3）或多或少地形成;一个（σ1）的形成量非常少。衣壳多肽μ2是病毒粒子的主要组成部分，它不是一次基因产物，而是从μ1多肽中去除10%的质量后得到的，所有衣壳多肽的合成似乎是同步开始的。在感染周期的大部分时间（从3到9小时），它们的相对合成速率只有微小的变化，但变化很大，没有证据表明合成了任何病毒特异性的非衣壳多肽，还测量了10种呼肠孤病毒mRNA在细胞内转录的程度。转录的模式显着不同的不受控制的模式所表现出的coresin体外。一些mRNA种类被频繁地翻译，而另一些则很少被翻译。因此，呼肠孤病毒信息的转录和翻译都受到控制。在循环早期形成的核心多肽分子倾向于比后来形成的这种分子更大程度地被掺入，而对于包含外壳的多肽分子则相反。研究了衣壳多肽合成的动力学作为感染复数和孵育温度的函数，并确定了呼肠孤病毒感染对宿主蛋白质合成的影响;宿主蛋白质合成直到感染周期的后期才减少。
All reovirus capsid polypeptides are formed in measurable amounts in mouse L fibroblasts. Five of them (λ1, λ2, μ1, σ2 and σ3) are formed more or less abundantly; one (σ1) is formed in very small amounts. Capsid polypeptide μ2, the major constituent of the virion, is not a primary gene product, but is derived from polypeptide μ1 by removal of 10% of its mass.The synthesis of all capsid polypeptides appears to start synchronously. During much of the infection cycle (from 3 to 9 hours) only minor, though significant, changes in their relative rates of synthesis occur.No evidence was obtained for the synthesis of any virus-specified noncapsid polypeptides.The extent to which the ten individual species of reovirus mRNA are transcribed within the cell was also measured. The pattern of transcription differs markedly from the uncontrolled pattern exhibited by coresin vitro. Some mRNA species are clearly translated frequently, others very infrequently. Both transcription and translation of reovirus message is thus controlled.The incorporation of capsid polypeptides formed at various times during the infection cycle into virions was determined. Core polypeptide molecules which are formed early in the cycle tend to be incorporated to a greater extent than such molecules which are formed later on, while the reverse is true for polypeptide molecules comprising the outer shell.The kinetics of synthesis of capsid polypeptides was studied as a function of the multiplicity of infection and the temperature of incubation and the effect of reovirus infection on host protein synthesis was determined; host protein synthesis does not decrease until late during the infection cycle.