Identification of New miRNA-mRNA Networks in the Development of Non-syndromic Cleft Lip With or Without Cleft Palate.
Identification of New miRNA-mRNA Networks in the Development of Non-syndromic Cleft Lip With or Without Cleft Palate.
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鉴定新的miRNA-mRNA网络在非综合征性唇腭裂伴或不伴腭裂发展中的作用
DOI：
10.3389/fcell.2021.631057
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发表时间：
2021
影响因子：
5.5
通讯作者：
Pan Y
中科院分区：
文献类型：
--
作者：
Fu C;Lou S;Zhu G;Fan L;Yu X;Zhu W;Ma L;Wang L;Pan Y
目的：探讨非综合征性唇腭裂（NSCL/P）组织中新的microRNA（miRNA）-mRNA网络。材料与方法：从腭裂患者（GSE 47939）和鼠胚胎口面组织（GSE 20880）中选择重叠差异表达的miRNA（DEM）。接下来，通过Targetscan、miRDB和FIGHICH预测DEM的靶基因，并通过来自NSCL/P患者和对照（GSE 42589）、MGI、MalaCards和DECIPHER数据库的差异表达基因（DEG）进一步筛选。然后通过体外实验证实了结果。获取NSCL/P唇组织，检测miRNAs及其靶基因的表达。结果：Let-7 c-5 p和miR-193 a-3 p被鉴定为DEMs，其过表达抑制细胞增殖，促进细胞凋亡。PIGA和TGFB 2分别被证实为let-7 c-5 p和miR-193 a-3 p的靶点，并参与小鼠的颅面发育。在NSCL/P唇组织中，miRNA与mRNA的表达呈负相关。根据MGI、MalaCards和DECIPHER数据库，它们也与NSCL/P的发生相关。结论：Let-7 c-5 p-PIGA和miR-193 a-3 p-TGFB 2网络可能参与NSCL/P的发生发展。
Objective: To identify new microRNA (miRNA)-mRNA networks in non-syndromic cleft lip with or without cleft palate (NSCL/P). Materials and Methods: Overlapping differentially expressed miRNAs (DEMs) were selected from cleft palate patients (GSE47939) and murine embryonic orofacial tissues (GSE20880). Next, the target genes of DEMs were predicted by Targetscan, miRDB, and FUNRICH, and further filtered through differentially expressed genes (DEGs) from NSCL/P patients and controls (GSE42589), MGI, MalaCards, and DECIPHER databases. The results were then confirmed by in vitro experiments. NSCL/P lip tissues were obtained to explore the expression of miRNAs and their target genes. Results: Let-7c-5p and miR-193a-3p were identified as DEMs, and their overexpression inhibited cell proliferation and promoted cell apoptosis. PIGA and TGFB2 were confirmed as targets of let-7c-5p and miR-193a-3p, respectively, and were involved in craniofacial development in mice. Negative correlation between miRNA and mRNA expression was detected in the NSCL/P lip tissues. They were also associated with the occurrence of NSCL/P based on the MGI, MalaCards, and DECIPHER databases. Conclusions: Let-7c-5p-PIGA and miR-193a-3p-TGFB2 networks may be involved in the development of NSCL/P.
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影响因子：
14.9
作者：
Rappaport N;Twik M;Plaschkes I;Nudel R;Iny Stein T;Levitt J;Gershoni M;Morrey CP;Safran M;Lancet D
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