Changes in polyamines, proline and ethylene in sunflower calluses treated with NaCl
Changes in polyamines, proline and ethylene in sunflower calluses treated with NaCl
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DOI：
10.1023/a:1023302012208
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发表时间：
2003-07-01
影响因子：
3
通讯作者：
Benavides, MP
中科院分区：
文献类型：
--
作者：
Alvarez, I;Tomaro, ML;Benavides, MP
研究了向日葵组织在对照 (C)、盐胁迫 (S) 和盐适应 (T) 愈伤组织中脯氨酸、多胺和乙烯含量方面对 NaCl 胁迫的反应，为期 21 天。盐适应愈伤组织通过在含有 175 mM NaCl 的培养基中生长来表现出对盐度的适应，其生长速度与 C 愈伤组织在无盐培养基上的生长速率相似。与第 0 时刻的对照愈伤组织相比，盐适应愈伤组织中的脯氨酸浓度高出 27 倍，但盐胁迫愈伤组织（S 愈伤组织）能够在第 21 天增加脯氨酸，这表明脯氨酸不仅是渗透压调节剂，而且可能参与向日葵盐胁迫愈伤组织中的其他反应。腐胺 (Put) 是时间 0 时 C 愈伤组织中最丰富的多胺，而亚精胺 (Spd) 是耐盐 (T) 愈伤组织中的主要多胺。直到第 14 天，C 愈伤组织中的乙烯含量不断增加，此后逐渐减少。在实验结束时，在盐适应愈伤组织中，乙烯浓度显着高于 C 和 S 愈伤组织中的浓度。在对照愈伤组织中，在第 21 天发现总多胺水平最高，乙烯水平最低，而此时 T 愈伤组织合成的乙烯水平最高，而多胺水平较低。在盐适应愈伤组织中，乙烯似乎与应激耐受性有关，而在盐敏感组织（S愈伤组织）中，乙烯形成与衰老有关。目前的数据表明向日葵去分化组织中脯氨酸、多胺、乙烯和盐胁迫耐受性之间存在密切关系。
Responses of sunflower tissues to NaCl stress were studied in control (C), salt-stressed (S) and salt-adapted (T) calluses in terms of proline, polyamines and ethylene content for a period of 21 days. Salt-adapted calluses showed their adaptation to salinity by growing in the medium with 175 mM NaCl, at a similar rate than C calluses on medium without salt. Proline concentration was 27 times higher in salt-adapted calluses compared to control calluses at time 0, but salt stressed calluses (S calluses) were able to increase proline by day 21, demonstrating that proline was not just an osmoregulator but might be involved in other responses in sunflower salt-stressed calluses. Putrescine (Put) was the most abundant polyamine in C calluses at time 0, while spermidine (Spd) was the main polyamine in salt tolerant (T) calluses. Ethylene increased in C calluses until day 14, decreasing thereafter. In salt-adapted calluses, ethylene increased significantly over the concentration in C and S calluses by the end of the experiment. In control calluses, the highest level of total polyamines and the lowest of ethylene was found on day 21, while T calluses synthesized the highest ethylene level and had the lower polyamines level by this time. It seems that in salt-adapted calluses ethylene was related to stress tolerance and in salt sensitive tissues (S calluses), ethylene formation was related to senescence. The present data suggests a close relationship between proline, polyamines, ethylene and salt-stress tolerance in sunflower dedifferentiated tissues.