In vitro antimutagenic, cytotoxic and anticancer potential of Fagonia indica phytochemicals
In vitro antimutagenic, cytotoxic and anticancer potential of Fagonia indica phytochemicals
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DOI：
10.36721/pjps.2021.34.6.sup.2325-2331.1
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发表时间：
2021-11-01
影响因子：
0.8
通讯作者：
Riaz, Sana
中科院分区：
文献类型：
--
作者：
Khalid, Samrah;Tiwana, Hira;Riaz, Sana
癌症是世界上诊断最多、威胁生命的疾病之一。然而，目前对癌症的临床治疗是手术和放疗，这不足以控制疾病负担。在过去的二十年中，超过一半的化疗药物直接或间接依赖于药用基础植物化合物或其衍生物。本研究旨在为植物化学药物的化学治疗提供基础。结果表明，在对照试验中，鸢尾的IC50值分别为146.33 +/- 5.2 μ g/ml、TA100 (AZS) 105.33 +/- 4.0 μ g/ml、TA98 (2AA) 113.6 +/- 5.2 μ g/ml和TA98 (AZS) 112.6 +/- 4.4 μ g/ml。为此，采用瑞祖脲荧光法研究了其提取物对癌细胞的抗增殖作用。在HepG-2细胞株上记录到（FIWM） 50%的细胞毒浓度（CC50）为128.3 +/-,2.43 μ g/ml。在智人肺组织上皮细胞A549上，高通量仪器分析表明，Fagonia indica在30hr内的细胞毒作用最大。电阻抗显示定性细胞凋亡表达的实时证据。在硅分子对接研究中，阻抗结果得到了来自Fagonia indica的棕榈酸的支持，棕榈酸实际上抑制了细胞周期蛋白依赖性激酶2 （cdk2）。稻瘟病菌提取物具有大量的抗诱变、细胞毒和抗癌活性，这支持了其植物化学物质在药物开发中的潜力。
Cancer is one of the most diagnosed and life threatening disease throughout the world. Nevertheless present day clinical management for cancers are surgery, radiations which are insufficient to contain the disease burden. In the past two decades, more than half of chemotherapeutic drugs developed are either directly or indirectly dependent on medicinal base phytocompounds or their derivative. The present study aims to provide the base for chemotherapeutic phytochemicals. Fagonia indica showed significant antimutagenic potential with reference to control IC50 values were calculated as 146.33 +/- 5.2 mu g/ml, TA100 (AZS) 105.33 +/- 4.0 mu g/ml, TA98 (2AA) 113.6 +/- 5.2 mu g/ml followed and TA98 (AZS) 112.6 +/- 4.4 in Ames test. For this reason, the antiproliferation effect of extracts on cancer cell lines was studied through resazurin fluorescence. On HepG-2 cell lines 50% cytotoxic concentration (CC50) of (FIWM) was recorded as 128.3 +/-,2.43 mu g/ml. On the homo sapiens epithelial cell of lung tissue (A549), the high throughput instrumental analysis of Fagonia indica depicts maximum cytotoxic effect in 30hr. The electrical impedance displays the real-time evidence about qualitative apoptosis expressed. The impedance results were supported as palmitic acid from Fagonia indica virtually that inhibits Cyclin Dependent Kinase 2 (CDKs 2) in silico molecular docking studies. Fagonia indica extract possesses substantial antimutagenic, cytotoxic and anticancer activity which supports the potential of its phytochemicals for drug development.