Comparative Toxicosis of Sodium Selenite and Selenomethionine in Lambs
Comparative Toxicosis of Sodium Selenite and Selenomethionine in Lambs
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DOI：
10.1177/104063870601800108
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发表时间：
2006-01
影响因子：
1.5
通讯作者：
Asheesh K. Tiwary;B. Stegelmeier;K. Panter;L. James;Jeffery O. Hall
中科院分区：
文献类型：
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作者：
Asheesh K. Tiwary;B. Stegelmeier;K. Panter;L. James;Jeffery O. Hall
过量消耗硒 (Se) 蓄积植物会导致硒中毒。本文报道的研究目的是比较植物中有机硒（硒代蛋氨酸）引起的急性中毒与补充无机形式硒（亚硒酸钠）引起的急性中毒。羔羊口服单剂量的硒（亚硒酸钠或硒代蛋氨酸），并监测7天，之后对它们实施安乐死并进行尸检。 12 个随机分配的治疗组由给予 0、1、2、3 或 4 mg Se/kg 体重（亚硒酸钠）或 0、1、2、3、4、6 或 8 mg Se/kg（硒代蛋氨酸）的动物组成。剂量为 2、3 和 4 mg/kg 的亚硒酸钠以及剂量为 4、6 和 8 mg/kg 的硒代蛋氨酸会导致轻微运动后呼吸急促和/或呼吸窘迫。严重程度和恢复时间各不相同，并且具有剂量依赖性。高剂量组动物的主要组织病理学发现包括多灶性心肌坏死和肺泡血管炎伴肺水肿和出血。对肝脏、肾皮质、心脏、血液和血清的分析显示，硒浓度呈线性、剂量依赖性增加。然而，经硒代蛋氨酸处理的羔羊的组织硒浓度显着高于经同等剂量亚硒酸钠处理的羔羊。为了评估这些硒化合物在体内的氧化作用，还测量了肝脏维生素 E 浓度。施用亚硒酸钠而非硒代蛋氨酸会导致肝脏维生素 E 浓度降低。这项研究的结果表明，必须了解摄入的硒的化学形式，才能充分解释组织、血液和血清中的硒浓度。
Excess consumption of selenium (Se) accumulator plants can result in selenium intoxication. The objective of the study reported here was to compare the acute toxicosis caused by organic selenium (selenomethionine) found in plants with that caused by the supplemental, inorganic form of selenium (sodium selenite). Lambs were orally administered a single dose of selenium as either sodium selenite or selenomethionine and were monitored for 7 days, after which they were euthanized and necropsied. Twelve randomly assigned treatment groups consisted of animals given 0, 1, 2, 3, or 4 mg of Se/kg of body weight as sodium selenite, or 0, 1, 2, 3, 4, 6, or 8 mg of Se/kg as selenomethionine. Sodium selenite at dosages of 2, 3, and 4 mg/kg, as well as selenomethionine at dosages of 4, 6, and 8 mg/kg resulted in tachypnea and/or respiratory distress following minimal exercise. Severity and time to recovery varied, and were dose dependent. Major histopathologic findings in animals of the high-dose groups included multifocal myocardial necrosis and pulmonary alveolar vasculitis with pulmonary edema and hemorrhage. Analysis of liver, kidney cortex, heart, blood, and serum revealed linear, dose-dependent increases in selenium concentration. However, tissue selenium concentration in selenomethionine-treated lambs were significantly greater than that in lambs treated with equivalent doses of sodium selenite. To estimate the oxidative effects of these selenium compounds in vivo, liver vitamin E concentration also was measured. Sodium selenite, but not selenomethionine administration resulted in decreased liver vitamin E concentration. Results of this study indicate that the chemical form of the ingested Se must be known to adequately interpret tissue, blood, and serum Se concentrations.