INHIBITION EFFECTS OF SCORPION VENOM EXTRACTS (BUTHUS MATENSII KARSCH) ON THE GROWTH OF HUMAN BREAST CANCER MCF-7 CELLS
INHIBITION EFFECTS OF SCORPION VENOM EXTRACTS (BUTHUS MATENSII KARSCH) ON THE GROWTH OF HUMAN BREAST CANCER MCF-7 CELLS
复制标题
蝎毒提取物（buthus matensii karsch）对人乳腺癌MCF-7细胞生长的抑制作用
DOI：
10.4314/ajtcam.v11i5.17
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Mao, Weifeng
中科院分区：
文献类型：
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作者：
Li, Weiling;Li, Ye;Mao, Weifeng
背景资料：为观察东亚钳蝎蝎毒提取物对人乳腺癌MCF-7细胞生长的抑制作用并探讨其作用机制，采用MTT法检测两种常见的肿瘤细胞（SMMC 7721、MCF-7）对蝎毒的敏感性。流式细胞仪检测细胞周期。免疫细胞化学法检测细胞凋亡相关蛋白Caspase-3和Bcl-2的表达，Western blotting法检测细胞周期相关蛋白Cyclin D1的表达。蝎毒提取物能抑制MCF-7细胞的生长和增殖。蝎毒提取物通过上调Caspase-3、下调Bcl-2诱导MCF-7细胞凋亡。结论：BmK蝎毒提取物通过诱导MCF-7细胞凋亡，阻滞细胞周期于G（0）/G（1）期，从而抑制MCF-7细胞的生长。BmK蝎毒提取物对乳腺癌的治疗将是非常有价值的。
Background: To observe the inhibition effects of the Buthus matensii Karsch (BmK) scorpion venom extracts on the growth of human breast cancer MCF-7 cells, and to explore its mechanisms.Methods: Two common tumor cells (SMMC7721, MCF-7) were examined for the one which wasmore sensitivity to scorpion venom by MTT method. Cell cycle was determined by flow cytometry. Immunocytochemistry was applied to detect apoptosis-related protein Caspase-3 and Bcl-2 levels, while the expression of cell cycle-related protein Cyclin D1 was shown by Western blotting.Results: Our data indicated that MCF-7 was the more sensitive cell line to scorpion venom. The extracts of scorpion venom could inhibit the growth and proliferation of MCF-7 cells. Furthermore, the extract of scorpion venom induced apoptosis through Caspase-3 up-regulation while Bcl-2 down-regulation in MCF-7 cells. In addition, the extracts of scorpion venom blocked the cells from G(0)/G(1) phase to S phase and decreased cell cycle-related protein Cyclin D1 level after drug intervention compared with the negative control group.Conclusions: These results showed that the BmK scorpion venom extracts could inhibit the growth of MCF-7 cells by inducing apoptosis and blocking cell cycle in G(0)/G(1) phase. The BmK scorpion venom extracts will be very valuable for the treatment of breast cancer.