The biological characteristics of SARS-CoV-2 spike protein Pro330-Leu650
The biological characteristics of SARS-CoV-2 spike protein Pro330-Leu650
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DOI：
10.1016/j.vaccine.2020.04.070
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发表时间：
2020-07-06
期刊：
影响因子：
5.5
通讯作者：
Wu, Gui-zhen
中科院分区：
文献类型：
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作者：
Su, Qiu-dong;Yi, Yao;Wu, Gui-zhen
SARS-CoV-2是新冠肺炎在全球范围内爆发的原因，世卫组织已将其描述为一种大流行。自2019年12月31日新冠肺炎首次报告以来，截至2020年3月17日，在160个国家/地区确诊179,111例，死亡7,426例。然而，到目前为止，世界上还没有疫苗被批准。本研究利用生物统计学方法对SARS-CoV-2刺突蛋白Pro330-Leu650(SARS-CoV-2-SPL)的生物学特性进行了分析。SARS-CoV-2-SPL具有受体结合区(RBD)和重要的B细胞表位(Ser438-Gln506、Thr553-Glu583、Gly404-Aps427、Thr345-Ala352和Lys529-Lys535)和T(9个CD4和11个CD8 T细胞抗原决定簇)细胞表位。考虑到氨基酸的生物相似性，剔除受体结合基序(RBM)，SARS-CoV-2与SARS-CoV在该区域的同源性高达87.7%。总的拓扑结构表明，SARS-CoV-2-SPL的完整结构是以RBM为头部，RBD为主干和尾部。SARS-CoV-2-SPL被发现具有诱导有效的B和T细胞应答的潜力。我们的研究结果可能为SARS-CoV-2疫苗的设计提供有意义的指导。(C)2020爱思唯尔有限公司。保留所有权利。
SARS-CoV-2 is the cause of the worldwide outbreak of COVID-19 that has been characterized as a pandemic by the WHO. Since the first report of COVID-19 on December 31, 2019, 179,111 cases were confirmed in 160 countries/regions with 7426 deaths as of March 17, 2020. However, there have been no vaccines approved in the world to date. In this study, we analyzed the biological characteristics of the SARS-CoV-2 Spike protein, Pro330-Leu650 (SARS-CoV-2-SPL), using biostatistical methods. SARS-CoV-2-SPL possesses a receptor-binding region (RBD) and important B (Ser438-Gln506, Thr553-Glu583, Gly404-Aps427, Thr345-Ala352, and Lys529-Lys535) and T (9 CD4 and 11 CD8 T cell antigenic determinants) cell epitopes. High homology in this region between SARS-CoV-2 and SARS-CoV amounted to 87.7%, after taking the biological similarity of the amino acids into account and eliminating the receptor-binding motif (RBM). The overall topology indicated that the complete structure of SARS-CoV-2-SPL was with RBM as the head, and RBD as the trunk and the tail region. SARS-CoV-2-SPL was found to have the potential to elicit effective B and T cell responses. Our findings may provide meaningful guidance for SARS-CoV-2 vaccine design. (C) 2020 Elsevier Ltd. All rights reserved.